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Title: Apparatus for improved DNA sequencing

This invention is a means for the rapid sequencing of DNA samples. More specifically, it consists of a new design direct blotting electrophoresis unit. The DNA sequence is deposited on a membrane attached to a rotating drum. Initial data compaction is facilitated by the use of a machined multi-channeled plate called a ribbon channel plate. Each channel is an isolated mini gel system much like a gel filled capillary. The system as a whole, however, is in a slab gel like format with the advantages of uniformity and easy reusability. The system can be used in different embodiments. The drum system is unique in that after deposition the drum rotates the deposited DNA into a large non-buffer open space where processing and detection can occur. The drum can also be removed in toto to special workstations for downstream processing, multiplexing and detection.
Inventors:
 [1];  [2]
  1. (Richland, WA)
  2. (Eltopia, WA)
Issue Date:
OSTI Identifier:
870402
Assignee:
Battelle Memorial Institute (Richland, WA) PNNL
Patent Number(s):
US 5514256
Contract Number:
AC06-76RL01830
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA
Country of Publication:
United States
Language:
English
Subject:
apparatus; improved; dna; sequencing; means; rapid; samples; specifically; consists; design; direct; blotting; electrophoresis; unit; sequence; deposited; membrane; attached; rotating; drum; initial; data; compaction; facilitated; machined; multi-channeled; plate; called; ribbon; channel; isolated; mini; filled; capillary; slab; format; advantages; uniformity; easy; reusability; embodiments; unique; deposition; rotates; non-buffer; space; processing; detection; occur; removed; toto; special; workstations; downstream; multiplexing; channel plate; dna sequence; dna sequencing; rotating drum; initial data; data compaction; /204/