Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions
Abstract
Compounds and methods for single step, covalent labeling of the phosphate group of an organic substance under aqueous conditions are described. The labeling compound includes any kind of detectable signal group covalently bound to an imidazole moiety, which can be imidazole or a substituted imidazole. A preferred labeling compound has the formula ##STR1##
- Inventors:
- Issue Date:
- Research Org.:
- Northeastern Univ., Boston, MA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 870396
- Patent Number(s):
- 5512486
- Application Number:
- 08/060,569
- Assignee:
- Northeastern University (Boston, MA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07F - ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- FG02-90ER60964
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 1993 May 10
- Country of Publication:
- United States
- Language:
- English
- Subject:
- single; step; signal; group-imidazole; labeling; organic; phosphate; aqueous; conditions; compounds; methods; covalent; substance; described; compound; detectable; covalently; bound; imidazole; moiety; substituted; preferred; formula; str1; covalently bound; single step; organic phosphate; organic substance; aqueous conditions; /436/999/
Citation Formats
Giese, Roger W., and Wang, Poguang. Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions. United States: N. p., 1996.
Web.
Giese, Roger W., & Wang, Poguang. Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions. United States.
Giese, Roger W., and Wang, Poguang. Mon .
"Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions". United States. https://www.osti.gov/servlets/purl/870396.
@article{osti_870396,
title = {Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions},
author = {Giese, Roger W. and Wang, Poguang},
abstractNote = {Compounds and methods for single step, covalent labeling of the phosphate group of an organic substance under aqueous conditions are described. The labeling compound includes any kind of detectable signal group covalently bound to an imidazole moiety, which can be imidazole or a substituted imidazole. A preferred labeling compound has the formula ##STR1##},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}
Works referenced in this record:
Solution-phase detection of polynucleotides using interacting fluorescent labels and competitive hybridization
journal, December 1989
- Morrison, Larry E.; Halder, Thomas C.; Stols, Lucy M.
- Analytical Biochemistry, Vol. 183, Issue 2
Derivatization of unprotected polynucleotides
journal, January 1983
- Chu, Barbara C. F.; Wahl, Geoffrey M.; Orgel, Leslie E.
- Nucleic Acids Research, Vol. 11, Issue 18
Nitrogen-bound and carbon-bound imidazole complexes of ruthenium ammines
journal, January 1974
- Sundberg, Richard J.; Bryan, Robert F.; Taylor, Ivan F.
- Journal of the American Chemical Society, Vol. 96, Issue 2
Preparation of ethylenediaminephosphoramidates of nucleotides and derivatization with fluorescein isothiocyanate
journal, July 1990
- Al-Deen, Amr Nour; Cecchini, Douglas C.; Abdel-Baky, Samy
- Journal of Chromatography A, Vol. 512
Modification of Oligo (Poly) Nucleotide Phosphomonoester Groups in Aqueous Solutions
journal, December 1987
- Ivanovskaya, M. G.; Gottikh, M. B.; Shabarova, Z. A.
- Nucleosides and Nucleotides, Vol. 6, Issue 5