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Title: Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions

Abstract

Compounds and methods for single step, covalent labeling of the phosphate group of an organic substance under aqueous conditions are described. The labeling compound includes any kind of detectable signal group covalently bound to an imidazole moiety, which can be imidazole or a substituted imidazole. A preferred labeling compound has the formula ##STR1##

Inventors:
;
Issue Date:
Research Org.:
Northeastern Univ., Boston, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
870396
Patent Number(s):
5512486
Application Number:
08/060,569
Assignee:
Northeastern University (Boston, MA)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FG02-90ER60964
Resource Type:
Patent
Resource Relation:
Patent File Date: 1993 May 10
Country of Publication:
United States
Language:
English
Subject:
single; step; signal; group-imidazole; labeling; organic; phosphate; aqueous; conditions; compounds; methods; covalent; substance; described; compound; detectable; covalently; bound; imidazole; moiety; substituted; preferred; formula; str1; covalently bound; single step; organic phosphate; organic substance; aqueous conditions; /436/999/

Citation Formats

Giese, Roger W., and Wang, Poguang. Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions. United States: N. p., 1996. Web.
Giese, Roger W., & Wang, Poguang. Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions. United States.
Giese, Roger W., and Wang, Poguang. Mon . "Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions". United States. https://www.osti.gov/servlets/purl/870396.
@article{osti_870396,
title = {Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions},
author = {Giese, Roger W. and Wang, Poguang},
abstractNote = {Compounds and methods for single step, covalent labeling of the phosphate group of an organic substance under aqueous conditions are described. The labeling compound includes any kind of detectable signal group covalently bound to an imidazole moiety, which can be imidazole or a substituted imidazole. A preferred labeling compound has the formula ##STR1##},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}

Works referenced in this record:

Solution-phase detection of polynucleotides using interacting fluorescent labels and competitive hybridization
journal, December 1989


Derivatization of unprotected polynucleotides
journal, January 1983


Nitrogen-bound and carbon-bound imidazole complexes of ruthenium ammines
journal, January 1974


Preparation of ethylenediaminephosphoramidates of nucleotides and derivatization with fluorescein isothiocyanate
journal, July 1990


Modification of Oligo (Poly) Nucleotide Phosphomonoester Groups in Aqueous Solutions
journal, December 1987