Multiplexed fluorescence detector system for capillary electrophoresis
Abstract
A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.
- Inventors:
-
- Ames, IA
- Nevada, IA
- Issue Date:
- Research Org.:
- Ames Lab., Ames, IA (United States); Iowa State Univ., Ames, IA (United States)
- OSTI Identifier:
- 870334
- Patent Number(s):
- 5498324
- Application Number:
- 08/223,864
- Assignee:
- Iowa State University Research Foundation, Inc. (Ames, IA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-82
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- multiplexed; fluorescence; detector; capillary; electrophoresis; detection; provided; simultaneously; excite; substantially; monitor; separations; multiple; capillaries; multiplexing; approach; involves; laser; irradiation; sample; plurality; optical; fibers; coupled; individually; array; imaged; orthogonally; microscope; charge-coupled; device; camera; signal; analysis; fluorescence detection; charge-coupled device; multiple capillaries; laser irradiation; coupled device; substantially simultaneously; capillary electrophoresis; optical fibers; optical fiber; signal analysis; approach involves; fluorescence detector; simultaneously monitor; simultaneously excite; multiplexed fluorescence; device camera; excite fluorescence; substantially simultaneous; /204/356/
Citation Formats
Yeung, Edward S, and Taylor, John A. Multiplexed fluorescence detector system for capillary electrophoresis. United States: N. p., 1996.
Web.
Yeung, Edward S, & Taylor, John A. Multiplexed fluorescence detector system for capillary electrophoresis. United States.
Yeung, Edward S, and Taylor, John A. Mon .
"Multiplexed fluorescence detector system for capillary electrophoresis". United States. https://www.osti.gov/servlets/purl/870334.
@article{osti_870334,
title = {Multiplexed fluorescence detector system for capillary electrophoresis},
author = {Yeung, Edward S and Taylor, John A},
abstractNote = {A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}
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