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Title: Multiplexed fluorescence detector system for capillary electrophoresis

Abstract

A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.

Inventors:
 [1];  [2]
  1. Ames, IA
  2. Nevada, IA
Issue Date:
Research Org.:
Ames Lab., Ames, IA (United States); Iowa State Univ., Ames, IA (United States)
OSTI Identifier:
870334
Patent Number(s):
5498324
Application Number:
08/223,864
Assignee:
Iowa State University Research Foundation, Inc. (Ames, IA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
multiplexed; fluorescence; detector; capillary; electrophoresis; detection; provided; simultaneously; excite; substantially; monitor; separations; multiple; capillaries; multiplexing; approach; involves; laser; irradiation; sample; plurality; optical; fibers; coupled; individually; array; imaged; orthogonally; microscope; charge-coupled; device; camera; signal; analysis; fluorescence detection; charge-coupled device; multiple capillaries; laser irradiation; coupled device; substantially simultaneously; capillary electrophoresis; optical fibers; optical fiber; signal analysis; approach involves; fluorescence detector; simultaneously monitor; simultaneously excite; multiplexed fluorescence; device camera; excite fluorescence; substantially simultaneous; /204/356/

Citation Formats

Yeung, Edward S, and Taylor, John A. Multiplexed fluorescence detector system for capillary electrophoresis. United States: N. p., 1996. Web.
Yeung, Edward S, & Taylor, John A. Multiplexed fluorescence detector system for capillary electrophoresis. United States.
Yeung, Edward S, and Taylor, John A. Mon . "Multiplexed fluorescence detector system for capillary electrophoresis". United States. https://www.osti.gov/servlets/purl/870334.
@article{osti_870334,
title = {Multiplexed fluorescence detector system for capillary electrophoresis},
author = {Yeung, Edward S and Taylor, John A},
abstractNote = {A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}

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Works referenced in this record:

Applications of charge transfer devices in spectroscopy
journal, March 1988


Temperature programming in capillary zone electrophoresis
journal, March 1992


Gel-filled capillaries for nucleic acid separations in capillary electrophoresis
journal, September 1992


DNA sequencing using capillary array electrophoresis
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Three DNA sequencing methods using capillary gel electrophoresis and laser-induced fluorescence
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DNA sequencing, automation, and the human genome
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Facilitating data transfer and improving precision in capillary zone electrophoresis with migration indexes
journal, December 1991


Fluorescence detection in capillary zone electrophoresis using a charge-coupled device with time-delayed integration
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Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.
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High-performance charge transfer device detectors
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Native fluorescence detection of nucleic acids and DNA restriction fragments in capillary electrophoresis
journal, January 1993


Capillary array electrophoresis using laser-excited confocal fluorescence detection
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Axial-beam laser-excited fluorescence detection in capillary electrophoresis
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Multiplex DNA sequencing
journal, April 1988