Multiple sort flow cytometer
Abstract
A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane.
- Inventors:
-
- Seattle, WA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 870251
- Patent Number(s):
- 5483469
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- multiple; sort; flow; cytometer; utilizes; lasers; excitation; respective; fluorescence; identified; dyes; bonded; specific; cells; events; identify; verify; sorted; sheath; droplet; stream; verified; timed; event; independently; tagged; separation; electrical; charge; 60; 120; 180; volts; passed; oppositely; charged; deflection; plates; ground; planes; yield; focused; six; narrow; plane; sheath flow; electrical charge; ground plane; flow cytometer; specific cells; multiple laser; ground planes; multiple lasers; droplet stream; deflection plate; /702/209/324/356/435/436/
Citation Formats
Van den Engh, Ger, and Esposito, Richard J. Multiple sort flow cytometer. United States: N. p., 1996.
Web.
Van den Engh, Ger, & Esposito, Richard J. Multiple sort flow cytometer. United States.
Van den Engh, Ger, and Esposito, Richard J. Mon .
"Multiple sort flow cytometer". United States. https://www.osti.gov/servlets/purl/870251.
@article{osti_870251,
title = {Multiple sort flow cytometer},
author = {Van den Engh, Ger and Esposito, Richard J},
abstractNote = {A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1996},
month = {Mon Jan 01 00:00:00 EST 1996}
}
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