Multiple sort flow cytometer

Van den Engh, Ger; Esposito, Richard J

Title: Multiple sort flow cytometer

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Abstract

A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane.

Inventors:

Van den Engh, Ger ^[1]; Esposito, Richard J ^[1]

Seattle, WA

Issue Date:: 1996-01-01

Research Org.:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

OSTI Identifier:: 870251

Patent Number(s):: 5483469

Assignee:: Regents of University of California (Oakland, CA)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2015/149 - {Sorting the particles}
G01N15/14 - Electro-optical investigation, e.g. flow cytometers

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DOE Contract Number:: W-7405-ENG-48

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: multiple; sort; flow; cytometer; utilizes; lasers; excitation; respective; fluorescence; identified; dyes; bonded; specific; cells; events; identify; verify; sorted; sheath; droplet; stream; verified; timed; event; independently; tagged; separation; electrical; charge; 60; 120; 180; volts; passed; oppositely; charged; deflection; plates; ground; planes; yield; focused; six; narrow; plane; sheath flow; electrical charge; ground plane; flow cytometer; specific cells; multiple laser; ground planes; multiple lasers; droplet stream; deflection plate; /702/209/324/356/435/436/

Citation Formats


                    Van den Engh, Ger, and Esposito, Richard J. Multiple sort flow cytometer.  United States: N. p., 1996. 
        Web.

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                    Van den Engh, Ger, & Esposito, Richard J. Multiple sort flow cytometer.  United States.

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                    Van den Engh, Ger, and Esposito, Richard J. Mon .  
        "Multiple sort flow cytometer".  United States.  https://www.osti.gov/servlets/purl/870251.

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@article{osti_870251,

  title        = {Multiple sort flow cytometer},

  author       = {Van den Engh, Ger and Esposito, Richard J},

  abstractNote = {A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1996},

  month        = {1}

}

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