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Title: Multiple sort flow cytometer

A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane.
Inventors:
 [1];  [1]
  1. (Seattle, WA)
Issue Date:
OSTI Identifier:
870251
Assignee:
Regents of University of California (Oakland, CA) LLNL
Patent Number(s):
US 5483469
Contract Number:
W-7405-ENG-48
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Country of Publication:
United States
Language:
English
Subject:
multiple; sort; flow; cytometer; utilizes; lasers; excitation; respective; fluorescence; identified; dyes; bonded; specific; cells; events; identify; verify; sorted; sheath; droplet; stream; verified; timed; event; independently; tagged; separation; electrical; charge; 60; 120; 180; volts; passed; oppositely; charged; deflection; plates; ground; planes; yield; focused; six; narrow; plane; sheath flow; electrical charge; ground plane; flow cytometer; specific cells; multiple laser; ground planes; multiple lasers; droplet stream; deflection plate; /702/209/324/356/435/436/