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Title: Multiple sort flow cytometer

Abstract

A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane.

Inventors:
 [1];  [1]
  1. Seattle, WA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
870251
Patent Number(s):
5483469
Assignee:
Regents of University of California (Oakland, CA)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
multiple; sort; flow; cytometer; utilizes; lasers; excitation; respective; fluorescence; identified; dyes; bonded; specific; cells; events; identify; verify; sorted; sheath; droplet; stream; verified; timed; event; independently; tagged; separation; electrical; charge; 60; 120; 180; volts; passed; oppositely; charged; deflection; plates; ground; planes; yield; focused; six; narrow; plane; sheath flow; electrical charge; ground plane; flow cytometer; specific cells; multiple laser; ground planes; multiple lasers; droplet stream; deflection plate; /702/209/324/356/435/436/

Citation Formats

Van den Engh, Ger, and Esposito, Richard J. Multiple sort flow cytometer. United States: N. p., 1996. Web.
Van den Engh, Ger, & Esposito, Richard J. Multiple sort flow cytometer. United States.
Van den Engh, Ger, and Esposito, Richard J. Mon . "Multiple sort flow cytometer". United States. https://www.osti.gov/servlets/purl/870251.
@article{osti_870251,
title = {Multiple sort flow cytometer},
author = {Van den Engh, Ger and Esposito, Richard J},
abstractNote = {A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}

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