Ethanol production in Gram-positive microbes
Abstract
The subject invention concerns the transformation of Gram-positive bacteria with heterologous genes which confer upon these microbes the ability to produce ethanol as a fermentation product. Specifically exemplified is the transformation of bacteria with genes, obtainable from Zymomonas mobilis, which encode pyruvate decarboxylase and alcohol dehydrogenase.
- Inventors:
-
- Gainesville, FL
- Issue Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- OSTI Identifier:
- 870245
- Patent Number(s):
- 5482846
- Assignee:
- University of Florida (Gainesville, FL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG05-86ER13574
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- ethanol; production; gram-positive; microbes; subject; concerns; transformation; bacteria; heterologous; genes; confer; ability; produce; fermentation; product; specifically; exemplified; obtainable; zymomonas; mobilis; encode; pyruvate; decarboxylase; alcohol; dehydrogenase; fermentation product; zymomonas mobilis; ethanol production; alcohol dehydrogenase; produce ethanol; pyruvate decarboxylase; specifically exemplified; heterologous genes; gram-positive bacteria; gram-positive microbes; /435/
Citation Formats
Ingram, Lonnie O'Neal, and Barbosa-Alleyne, Maria D. F. Ethanol production in Gram-positive microbes. United States: N. p., 1996.
Web.
Ingram, Lonnie O'Neal, & Barbosa-Alleyne, Maria D. F. Ethanol production in Gram-positive microbes. United States.
Ingram, Lonnie O'Neal, and Barbosa-Alleyne, Maria D. F. Mon .
"Ethanol production in Gram-positive microbes". United States. https://www.osti.gov/servlets/purl/870245.
@article{osti_870245,
title = {Ethanol production in Gram-positive microbes},
author = {Ingram, Lonnie O'Neal and Barbosa-Alleyne, Maria D. F.},
abstractNote = {The subject invention concerns the transformation of Gram-positive bacteria with heterologous genes which confer upon these microbes the ability to produce ethanol as a fermentation product. Specifically exemplified is the transformation of bacteria with genes, obtainable from Zymomonas mobilis, which encode pyruvate decarboxylase and alcohol dehydrogenase.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1996},
month = {Mon Jan 01 00:00:00 EST 1996}
}
Works referenced in this record:
Production of ethanol byClostridium thermosaccharolyticum: II. A quantitative model describing product distributions
journal, December 1989
- Mistry, Firoz R.; Cooney, Charles L.
- Biotechnology and Bioengineering, Vol. 34, Issue 10
Cloning and expression of the structural gene for pyruvate decarboxylase of Zymomonas mobilis in Escherichia coli
journal, April 1986
- Br�u, Barbara; Sahm, Hermann
- Archives of Microbiology, Vol. 144, Issue 3
Ethanol tolerance and carbohydrate metabolism in lactobacilli
journal, July 1992
- Gold, R. Shane; Meagher, M. M.; Hutkins, R.
- Journal of Industrial Microbiology, Vol. 10, Issue 1
Production of ethanol byClostridium thermosaccharolyticum: I. Effect of cell recycle and environmental parameters
journal, December 1989
- Mistry, Firoz R.; Cooney, Charles L.
- Biotechnology and Bioengineering, Vol. 34, Issue 10
Nucleotide sequence of the pyruvate decarboxylase gene from Zymomonas mobilis
journal, January 1987
- Neale, Alan D.; Scopes, Robert K.; Wettenhall, Richard E. H.
- Nucleic Acids Research, Vol. 15, Issue 4
Ethanol production by recombinantescherichia coli carrying genes from zymomonas mobilis
journal, March 1991
- Lawford, Hugh G.; Rousseau, Joyce D.
- Applied Biochemistry and Biotechnology, Vol. 28-29, Issue 1
Production of Ethanol from d-Xylose by Using d-Xylose Isomerase and Yeasts
journal, January 1981
- Gong, Cheng-Shung; Chen, Li-Fu; Flickinger, Michael C.
- Applied and Environmental Microbiology, Vol. 41, Issue 2
Expression of a xylanase gene of Bacillus pumilus in Escherichia coli and Bacillus subtilis
journal, August 1985
- Panbangred, Watanalai; Fukusaki, Eiichiro; Epifanio, EvangelineC.
- Applied Microbiology and Biotechnology, Vol. 22, Issue 4
Genetic engineering of ethanol production in Escherichia coli.
journal, January 1987
- Ingram, L. O.; Conway, T.; Clark, D. P.
- Applied and Environmental Microbiology, Vol. 53, Issue 10
Expression of theZymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes inBacillus
journal, May 1994
- Barbosa, Maria de F. S.; Ingram, L. O.
- Current Microbiology, Vol. 28, Issue 5
Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose
journal, May 1993
- Beall, D. S.; Ingram, L. O.
- Journal of Industrial Microbiology, Vol. 11, Issue 3
Pyruvate decarboxylase from Zymomonas mobilis. Isolation and partial characterization
journal, November 1986
- Bringer-Meyer, S.; Schimz, K. -L.; Sahm, H.
- Archives of Microbiology, Vol. 146, Issue 2