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Title: Method for construction of normalized cDNA libraries

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.
Inventors:
 [1];  [2]
  1. (New York, NY)
  2. (Englewood, NJ)
Issue Date:
OSTI Identifier:
870244
Assignee:
Trustees of Columbia University in City of New York (New York, NY) CHO
Patent Number(s):
US 5482845
Contract Number:
FG02-91ER61233
Research Org:
Columbia University
Country of Publication:
United States
Language:
English
Subject:
method; construction; normalized; cdna; libraries; provides; normalize; directional; library; constructed; vector; allows; propagation; single-stranded; circle; form; comprising; propagating; circles; generating; fragments; complementary; noncoding; sequence; produce; partial; duplexes; purifying; melting; reassociating; purified; moderate; cot; unassociated; cdna libraries; produce partial; directional cdna; single-stranded circles; cdna library; partial duplexes; normalized cdna; purified partial; single-stranded circle; unassociated single-stranded; noncoding sequence; circle form; allows propagation; coding sequence; library constructed; form comprising; generating fragments; fragments complementary; /435/999/

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