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Title: Method using CO for extending the useful shelf-life of refrigerated red blood cells

Method using CO for extending the useful shelf-life of refrigerated red blood cells. Carbon monoxide is utilized for stabilizing hemoglobin in red blood cells to be stored at low temperature. Changes observed in the stored cells are similar to those found in normal red cell aging in the body, the extent thereof being directly related to the duration of refrigerated storage. Changes in cell buoyant density, vesiculation, and the tendency of stored cells to bind autologous IgG antibody directed against polymerized band 3 IgG, all of which are related to red blood cell senescence and increase with refrigerated storage time, have been substantially slowed when red blood cells are treated with CO. Removal of the carbon monoxide from the red blood cells is readily and efficiently accomplished by photolysis in the presence of oxygen so that the stored red blood cells may be safely transfused into a recipient.
Inventors:
 [1]
  1. (Los Alamos, NM)
Issue Date:
OSTI Identifier:
870210
Assignee:
Regents of University of California (Los Alamos, NM) LANL
Patent Number(s):
US 5476764
Contract Number:
W-7405-ENG-36
Research Org:
Los Alamos National Laboratory (LANL), Los Alamos, NM
Country of Publication:
United States
Language:
English
Subject:
method; extending; useful; shelf-life; refrigerated; blood; cells; carbon; monoxide; utilized; stabilizing; hemoglobin; stored; temperature; changes; observed; similar; found; normal; cell; aging; extent; directly; related; duration; storage; buoyant; density; vesiculation; tendency; bind; autologous; igg; antibody; directed; polymerized; band; senescence; increase; time; substantially; slowed; treated; removal; readily; efficiently; accomplished; photolysis; presence; oxygen; safely; transfused; recipient; cell senescence; directly related; blood cells; carbon monoxide; blood cell; useful shelf-life; efficiently accomplished; /435/424/