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Title: Aqueous carrier waveguide in a flow cytometer

Abstract

The liquid of a flow cytometer itself acts as an optical waveguide, thus transmitting the light to an optical filter/detector combination. This alternative apparatus and method for detecting scattered light in a flow cytometer is provided by a device which views and detects the light trapped within the optical waveguide formed by the flow stream. A fiber optic or other light collecting device is positioned within the flow stream. This provides enormous advantages over the standard light collection technique which uses a microscope objective. The signal-to-noise ratio is greatly increased over that for right-angle-scattered light collected by a microscope objective, and the alignment requirements are simplified.

Inventors:
 [1];  [2];  [3]
  1. (Danville, CA)
  2. (Seattle, WA)
  3. (Berkeley, CA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
870205
Patent Number(s):
5475487
Assignee:
Regents of University of California (Oakland, CA) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
aqueous; carrier; waveguide; flow; cytometer; liquid; optical; transmitting; light; filter; detector; combination; alternative; apparatus; method; detecting; scattered; provided; device; views; detects; trapped; formed; stream; fiber; optic; collecting; positioned; provides; enormous; advantages; standard; collection; technique; microscope; objective; signal-to-noise; ratio; greatly; increased; right-angle-scattered; collected; alignment; requirements; simplified; carrier wave; signal-to-noise ratio; optical filter; scattered light; fiber optic; optical waveguide; flow cytometer; greatly increased; flow stream; noise ratio; optical wave; microscope objective; waveguide formed; light collection; right-angle-scattered light; light collected; greatly increase; detecting scattered; detector combination; /356/250/385/

Citation Formats

Mariella, Jr., Raymond P., van den Engh, Gerrit, and Northrup, M. Allen. Aqueous carrier waveguide in a flow cytometer. United States: N. p., 1995. Web.
Mariella, Jr., Raymond P., van den Engh, Gerrit, & Northrup, M. Allen. Aqueous carrier waveguide in a flow cytometer. United States.
Mariella, Jr., Raymond P., van den Engh, Gerrit, and Northrup, M. Allen. Sun . "Aqueous carrier waveguide in a flow cytometer". United States. https://www.osti.gov/servlets/purl/870205.
@article{osti_870205,
title = {Aqueous carrier waveguide in a flow cytometer},
author = {Mariella, Jr., Raymond P. and van den Engh, Gerrit and Northrup, M. Allen},
abstractNote = {The liquid of a flow cytometer itself acts as an optical waveguide, thus transmitting the light to an optical filter/detector combination. This alternative apparatus and method for detecting scattered light in a flow cytometer is provided by a device which views and detects the light trapped within the optical waveguide formed by the flow stream. A fiber optic or other light collecting device is positioned within the flow stream. This provides enormous advantages over the standard light collection technique which uses a microscope objective. The signal-to-noise ratio is greatly increased over that for right-angle-scattered light collected by a microscope objective, and the alignment requirements are simplified.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}

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