Aqueous carrier waveguide in a flow cytometer
Abstract
The liquid of a flow cytometer itself acts as an optical waveguide, thus transmitting the light to an optical filter/detector combination. This alternative apparatus and method for detecting scattered light in a flow cytometer is provided by a device which views and detects the light trapped within the optical waveguide formed by the flow stream. A fiber optic or other light collecting device is positioned within the flow stream. This provides enormous advantages over the standard light collection technique which uses a microscope objective. The signal-to-noise ratio is greatly increased over that for right-angle-scattered light collected by a microscope objective, and the alignment requirements are simplified.
- Inventors:
-
- (Danville, CA)
- Seattle, WA
- Berkeley, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 870205
- Patent Number(s):
- 5475487
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- aqueous; carrier; waveguide; flow; cytometer; liquid; optical; transmitting; light; filter; detector; combination; alternative; apparatus; method; detecting; scattered; provided; device; views; detects; trapped; formed; stream; fiber; optic; collecting; positioned; provides; enormous; advantages; standard; collection; technique; microscope; objective; signal-to-noise; ratio; greatly; increased; right-angle-scattered; collected; alignment; requirements; simplified; carrier wave; signal-to-noise ratio; optical filter; scattered light; fiber optic; optical waveguide; flow cytometer; greatly increased; flow stream; noise ratio; optical wave; microscope objective; waveguide formed; light collection; right-angle-scattered light; light collected; greatly increase; detecting scattered; detector combination; /356/250/385/
Citation Formats
Mariella, Jr., Raymond P., van den Engh, Gerrit, and Northrup, M Allen. Aqueous carrier waveguide in a flow cytometer. United States: N. p., 1995.
Web.
Mariella, Jr., Raymond P., van den Engh, Gerrit, & Northrup, M Allen. Aqueous carrier waveguide in a flow cytometer. United States.
Mariella, Jr., Raymond P., van den Engh, Gerrit, and Northrup, M Allen. Sun .
"Aqueous carrier waveguide in a flow cytometer". United States. https://www.osti.gov/servlets/purl/870205.
@article{osti_870205,
title = {Aqueous carrier waveguide in a flow cytometer},
author = {Mariella, Jr., Raymond P. and van den Engh, Gerrit and Northrup, M Allen},
abstractNote = {The liquid of a flow cytometer itself acts as an optical waveguide, thus transmitting the light to an optical filter/detector combination. This alternative apparatus and method for detecting scattered light in a flow cytometer is provided by a device which views and detects the light trapped within the optical waveguide formed by the flow stream. A fiber optic or other light collecting device is positioned within the flow stream. This provides enormous advantages over the standard light collection technique which uses a microscope objective. The signal-to-noise ratio is greatly increased over that for right-angle-scattered light collected by a microscope objective, and the alignment requirements are simplified.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.