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Title: Colloidal-gold electrosensor measuring device

Abstract

The present invention provides a new device for use in measuring lead levels in biological and environmental samples. Using square wave coulometry and colloidal gold particles impregnated on carbon electrodes, the present invention provides a rapid, reliable, portable and inexpensive means of detecting low lead levels. The colloidal gold modified electrodes have microelectrode array characteristics and produce significantly higher stripping detection signals for lead than are produced at bulk gold electrode surfaces. The method is effective in determining levels of lead down to at least 5 .mu.g/dL in blood samples as small as 10 .mu.L.

Inventors:
 [1];  [2];  [2];  [1];  [3];  [1];  [2];  [2];  [4]
  1. Chapel Hill, NC
  2. Durham, NC
  3. Cary, NC
  4. Carrboro, NC
Issue Date:
Research Org.:
Andcare, Inc. (Durham, NC)
OSTI Identifier:
870163
Patent Number(s):
5468366
Assignee:
Andcare, Inc. (Durham, NC)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FG05-94ER81657
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
colloidal-gold; electrosensor; measuring; device; provides; lead; levels; biological; environmental; samples; square; wave; coulometry; colloidal; gold; particles; impregnated; carbon; electrodes; rapid; reliable; portable; inexpensive; means; detecting; modified; microelectrode; array; characteristics; produce; significantly; stripping; detection; signals; produced; bulk; electrode; surfaces; method; effective; determining; dl; blood; 10; carbon electrode; blood sample; blood samples; environmental sample; detection signals; electrode array; measuring device; electrode surface; environmental samples; square wave; carbon electrodes; microelectrode array; measuring lead; electrode surfaces; detection signal; inexpensive means; /205/204/435/436/

Citation Formats

Wegner, Steven, Harpold, Michael A, McCaffrey, Terence M, Morris, Susan E, Wojciechowski, Marek, Zhao, Junguo, Henkens, Robert W, Naser, Najih, and O'Daly, John P. Colloidal-gold electrosensor measuring device. United States: N. p., 1995. Web.
Wegner, Steven, Harpold, Michael A, McCaffrey, Terence M, Morris, Susan E, Wojciechowski, Marek, Zhao, Junguo, Henkens, Robert W, Naser, Najih, & O'Daly, John P. Colloidal-gold electrosensor measuring device. United States.
Wegner, Steven, Harpold, Michael A, McCaffrey, Terence M, Morris, Susan E, Wojciechowski, Marek, Zhao, Junguo, Henkens, Robert W, Naser, Najih, and O'Daly, John P. Sun . "Colloidal-gold electrosensor measuring device". United States. https://www.osti.gov/servlets/purl/870163.
@article{osti_870163,
title = {Colloidal-gold electrosensor measuring device},
author = {Wegner, Steven and Harpold, Michael A and McCaffrey, Terence M and Morris, Susan E and Wojciechowski, Marek and Zhao, Junguo and Henkens, Robert W and Naser, Najih and O'Daly, John P},
abstractNote = {The present invention provides a new device for use in measuring lead levels in biological and environmental samples. Using square wave coulometry and colloidal gold particles impregnated on carbon electrodes, the present invention provides a rapid, reliable, portable and inexpensive means of detecting low lead levels. The colloidal gold modified electrodes have microelectrode array characteristics and produce significantly higher stripping detection signals for lead than are produced at bulk gold electrode surfaces. The method is effective in determining levels of lead down to at least 5 .mu.g/dL in blood samples as small as 10 .mu.L.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}

Works referenced in this record:

Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes
journal, March 1985


Studies of protein adsorption on polystyrene latex surfaces
journal, October 1980


Inhibited enzyme electrodes. Part 3: A sensor for low levels of H2S and HCN
journal, January 1990


The conformation of γ-globulin adsorbed on polystyrene latices determined by quasielastic light scattering
journal, July 1978


The Reduction of Chlorine on Carbon in AlCl[sub 3]-KCl-NaCl Melts
journal, January 1972


Studies on acetylcholine sensor and its analytical application based on the inhibition of cholinesterase
journal, January 1990