High speed flow cytometric separation of viable cells
Abstract
Hematopoietic cell populations are separated to provide cell sets and subsets as viable cells with high purity and high yields, based on the number of original cells present in the mixture. High-speed flow cytometry is employed using light characteristics of the cells to separate the cells, where high flow speeds are used to reduce the sorting time.
- Inventors:
-
- Mountain View, CA
- Seattle, WA
- Margate, GB
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 870155
- Patent Number(s):
- 5466572
- Assignee:
- Systemix, Inc. (Palo Alto, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- speed; flow; cytometric; separation; viable; cells; hematopoietic; cell; populations; separated; provide; sets; subsets; purity; yields; based; original; mixture; high-speed; cytometry; employed; light; characteristics; separate; speeds; reduce; sorting; time; flow cytometric; flow cytometry; viable cells; cell populations; speed flow; /435/436/
Citation Formats
Sasaki, Dennis T, Van den Engh, Gerrit J, and Buckie, Anne-Marie. High speed flow cytometric separation of viable cells. United States: N. p., 1995.
Web.
Sasaki, Dennis T, Van den Engh, Gerrit J, & Buckie, Anne-Marie. High speed flow cytometric separation of viable cells. United States.
Sasaki, Dennis T, Van den Engh, Gerrit J, and Buckie, Anne-Marie. Sun .
"High speed flow cytometric separation of viable cells". United States. https://www.osti.gov/servlets/purl/870155.
@article{osti_870155,
title = {High speed flow cytometric separation of viable cells},
author = {Sasaki, Dennis T and Van den Engh, Gerrit J and Buckie, Anne-Marie},
abstractNote = {Hematopoietic cell populations are separated to provide cell sets and subsets as viable cells with high purity and high yields, based on the number of original cells present in the mixture. High-speed flow cytometry is employed using light characteristics of the cells to separate the cells, where high flow speeds are used to reduce the sorting time.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}
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Parallel processing data acquisition system for multilaser flow cytometry and cell sorting
journal, May 1989
- van den Engh, Ger; Stokdijk, Willem
- Cytometry, Vol. 10, Issue 3