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Title: High speed flow cytometric separation of viable cells

Abstract

Hematopoietic cell populations are separated to provide cell sets and subsets as viable cells with high purity and high yields, based on the number of original cells present in the mixture. High-speed flow cytometry is employed using light characteristics of the cells to separate the cells, where high flow speeds are used to reduce the sorting time.

Inventors:
 [1];  [2];  [3]
  1. (Mountain View, CA)
  2. (Seattle, WA)
  3. (Margate, GB)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
870155
Patent Number(s):
5466572
Assignee:
Systemix, Inc. (Palo Alto, CA) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
speed; flow; cytometric; separation; viable; cells; hematopoietic; cell; populations; separated; provide; sets; subsets; purity; yields; based; original; mixture; high-speed; cytometry; employed; light; characteristics; separate; speeds; reduce; sorting; time; flow cytometric; flow cytometry; viable cells; cell populations; speed flow; /435/436/

Citation Formats

Sasaki, Dennis T., Van den Engh, Gerrit J., and Buckie, Anne-Marie. High speed flow cytometric separation of viable cells. United States: N. p., 1995. Web.
Sasaki, Dennis T., Van den Engh, Gerrit J., & Buckie, Anne-Marie. High speed flow cytometric separation of viable cells. United States.
Sasaki, Dennis T., Van den Engh, Gerrit J., and Buckie, Anne-Marie. Sun . "High speed flow cytometric separation of viable cells". United States. https://www.osti.gov/servlets/purl/870155.
@article{osti_870155,
title = {High speed flow cytometric separation of viable cells},
author = {Sasaki, Dennis T. and Van den Engh, Gerrit J. and Buckie, Anne-Marie},
abstractNote = {Hematopoietic cell populations are separated to provide cell sets and subsets as viable cells with high purity and high yields, based on the number of original cells present in the mixture. High-speed flow cytometry is employed using light characteristics of the cells to separate the cells, where high flow speeds are used to reduce the sorting time.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}

Patent:

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