High speed flow cytometric separation of viable cells

Sasaki, Dennis T; Van den Engh, Gerrit J; Buckie, Anne-Marie

Title: High speed flow cytometric separation of viable cells

Abstract

Hematopoietic cell populations are separated to provide cell sets and subsets as viable cells with high purity and high yields, based on the number of original cells present in the mixture. High-speed flow cytometry is employed using light characteristics of the cells to separate the cells, where high flow speeds are used to reduce the sorting time.

Inventors:

Sasaki, Dennis T ^[1]; Van den Engh, Gerrit J ^[2]; Buckie, Anne-Marie ^[3]

Mountain View, CA
Seattle, WA
Margate, GB

Issue Date:: 1995-01-01

Research Org.:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

OSTI Identifier:: 870155

Patent Number(s):: 5466572

Assignee:: Systemix, Inc. (Palo Alto, CA)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2015/149 - {Sorting the particles}
G01N35/08 - using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

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DOE Contract Number:: W-7405-ENG-48

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: speed; flow; cytometric; separation; viable; cells; hematopoietic; cell; populations; separated; provide; sets; subsets; purity; yields; based; original; mixture; high-speed; cytometry; employed; light; characteristics; separate; speeds; reduce; sorting; time; flow cytometric; flow cytometry; viable cells; cell populations; speed flow; /435/436/

Citation Formats


                    Sasaki, Dennis T, Van den Engh, Gerrit J, and Buckie, Anne-Marie. High speed flow cytometric separation of viable cells.  United States: N. p., 1995. 
        Web.

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                    Sasaki, Dennis T, Van den Engh, Gerrit J, & Buckie, Anne-Marie. High speed flow cytometric separation of viable cells.  United States.

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                    Sasaki, Dennis T, Van den Engh, Gerrit J, and Buckie, Anne-Marie. Sun .  
        "High speed flow cytometric separation of viable cells".  United States.  https://www.osti.gov/servlets/purl/870155.

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@article{osti_870155,

  title        = {High speed flow cytometric separation of viable cells},

  author       = {Sasaki, Dennis T and Van den Engh, Gerrit J and Buckie, Anne-Marie},

  abstractNote = {Hematopoietic cell populations are separated to provide cell sets and subsets as viable cells with high purity and high yields, based on the number of original cells present in the mixture. High-speed flow cytometry is employed using light characteristics of the cells to separate the cells, where high flow speeds are used to reduce the sorting time.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1995},

  month        = {1}

}

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Works referenced in this record:

High-speed chromosome sorting
journal, October 1987

Gray, J.; Dean, P.; Fuscoe, J.
Science, Vol. 238, Issue 4825
https://doi.org/10.1126/science.2443974

The LLNL high-speed sorter: Design features, operational characteristics, and biological utility
journal, July 1985

Peters, D.; Branscomb, E.; Dean, P.
Cytometry, Vol. 6, Issue 4
https://doi.org/10.1002/cyto.990060404

Parallel processing data acquisition system for multilaser flow cytometry and cell sorting
journal, May 1989

van den Engh, Ger; Stokdijk, Willem
Cytometry, Vol. 10, Issue 3
https://doi.org/10.1002/cyto.990100307

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Title: High speed flow cytometric separation of viable cells

Abstract

Citation Formats

High-speed chromosome sorting journal, October 1987

The LLNL high-speed sorter: Design features, operational characteristics, and biological utility journal, July 1985

Parallel processing data acquisition system for multilaser flow cytometry and cell sorting journal, May 1989

High-speed chromosome sorting
journal, October 1987

The LLNL high-speed sorter: Design features, operational characteristics, and biological utility
journal, July 1985

Parallel processing data acquisition system for multilaser flow cytometry and cell sorting
journal, May 1989