skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methods for chromosome-specific staining

Abstract

Methods and compositions for chromosome-specific staining are provided. Compositions comprise heterogenous mixtures of labeled nucleic acid fragments having substantially complementary base sequences to unique sequence regions of the chromosomal DNA for which their associated staining reagent is specific. Methods include methods for making the chromosome-specific staining compositions of the invention, and methods for applying the staining compositions to chromosomes.

Inventors:
 [1];  [2]
  1. Livermore, CA
  2. Walnut Creek, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
870054
Patent Number(s):
5447841
Assignee:
Regents Of Univ. Of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
methods; chromosome-specific; staining; compositions; provided; comprise; heterogenous; mixtures; labeled; nucleic; acid; fragments; substantially; complementary; base; sequences; unique; sequence; regions; chromosomal; dna; associated; reagent; specific; applying; chromosomes; compositions comprise; base sequences; chromosomal dna; nucleic acid; base sequence; chromosome-specific staining; complementary base; substantially complementary; staining reagent; acid fragment; /435/436/999/

Citation Formats

Gray, Joe W, and Pinkel, Daniel. Methods for chromosome-specific staining. United States: N. p., 1995. Web.
Gray, Joe W, & Pinkel, Daniel. Methods for chromosome-specific staining. United States.
Gray, Joe W, and Pinkel, Daniel. Sun . "Methods for chromosome-specific staining". United States. https://www.osti.gov/servlets/purl/870054.
@article{osti_870054,
title = {Methods for chromosome-specific staining},
author = {Gray, Joe W and Pinkel, Daniel},
abstractNote = {Methods and compositions for chromosome-specific staining are provided. Compositions comprise heterogenous mixtures of labeled nucleic acid fragments having substantially complementary base sequences to unique sequence regions of the chromosomal DNA for which their associated staining reagent is specific. Methods include methods for making the chromosome-specific staining compositions of the invention, and methods for applying the staining compositions to chromosomes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}

Patent:

Save / Share:

Works referenced in this record:

Specific DNA sequence amplification in human neuroblastoma cells.
journal, September 1983


Isolation and characterization of a major tandem repeat family from the human X chromosome
journal, January 1983


Heterogeneity of chromosome 22 breakpoint in Philadelphia-positive (Ph+) acute lymphocytic leukemia.
journal, March 1986


Detection of species specific chromosomes in somatic cell hybrids
journal, November 1985


Report of the committee on structural chromosome changes in neoplasia
journal, January 1989


Partial purification and characterization of DNA from the human X chromosome.
journal, December 1979


General method for cloning amplified DNA by differential screening with genomic probes.
journal, May 1982


The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit β-globin DNA
journal, January 1981


Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
journal, October 1975


Detection of the Philadelphia chromosome in interphase nuclei
journal, January 1990


Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.
journal, December 1988


Autoradiographic Detection of Molecular Hybrids between rRNA and DNA in Tissue Sections
journal, March 1970


Rapid detection of human chromosome 21 aberrations by in situ hybridization.
journal, December 1988


In situ hybridization and translocation breakpoint mapping
journal, January 1985


What's new with hybridization in situ?
journal, January 1982


Fetal cells in the blood of pregnant women: detection and enrichment by fluorescence-activated cell sorting.
journal, March 1979


Detection of structural and numerical abnormalities in metaphase spreads and interphase nuclei using in situ hybridization
journal, September 1989


RNA-DNA Hybrids at the Cytological Level
journal, August 1969


Organization and evolution of the class I gene family in the major histocompatibility complex of the C57BL/10 mouse
journal, August 1984


Removal of repeated sequences from hybridisation probes
journal, January 1985


Localization of the human insulin gene to the distal end of the short arm of chromosome 11.
journal, July 1981


A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma
journal, October 1986


Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes
journal, April 1981


Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method.
journal, November 1984


Immunological method for mapping genes on Drosophila polytene chromosomes.
journal, July 1982


Is non-isotopic in situ hybridization finally coming of age?
journal, May 1990


Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization
journal, July 1981


Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.
journal, May 1986


Isolation of unique sequence human X chromosomal deoxyribonucleic acid
journal, May 1980


Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization
journal, December 1987


Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes
journal, January 1977


Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization
journal, September 1985


Cloned DNA probes regionally mapped to human Chromosome 21 and their use in determining the origin of nondisjunction
journal, January 1985


Structure of amplified DNA in different Syrian hamster cell lines resistant to N-(phosphonacetyl)-L-aspartate.
journal, November 1983


Detection of chromosome aberrations in interphase tumor nuclei by nonradioactive in situ hybridization
journal, October 1989


Detection of DNA sequences in nuclei in suspension by in situ hybridization and dual beam flow cytometry
journal, December 1985


Assignment of the structural gene coding for albumin to human chromosome 4
journal, January 1982


Distinctive chromosomal structures are formed very early in the amplification of CAD genes in Syrian hamster cells
journal, December 1990


Regional assignment of genes for human esterase D and retinoblastoma to chromosome band 13q14
journal, May 1980


Clinical Applications of DNA Probes in the Diagnosis of Genetic Diseases
journal, January 1987


Individual interphase chromosome domains revealed by in situ hybridization
journal, December 1985


Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes
journal, February 1984


Hereditary Renal-Cell Carcinoma Associated with a Chromosomal Translocation
journal, September 1979


Formation and Detection of Rna-Dna Hybrid Molecules in Cytological Preparations
journal, June 1969


Chromosomal and nuclear distribution of the HindIII 1.9-kb human DNA repeat segment
journal, December 1984


Human retinoblastoma susceptibility gene: genomic organization and analysis of heterozygous intragenic deletion mutants.
journal, April 1988


Cytogenetic Analysis by In Situ Hybridization with Fluorescently Labeled Nucleic Acid Probes
journal, January 1986


2-Acetylaminofluorene-modified probes for the indirect hybridocytochemical detection of specific nucleic acid sequences
journal, July 1984


A highly polymorphic locus in human DNA revealed by cosmid-derived probes.
journal, September 1985


Characterization of a cloned DNA sequence that is present at centromeres of all human autosomes and the X chromosome and shows polymorphic variation.
journal, August 1984


High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones
journal, January 1990


Detection of amplified dna sequences in human tumor cell lines by fluorescence in situ hybridization
journal, June 1992