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Title: Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus

Abstract

A purified low molecular weight .beta.-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-.beta.-D-glucopyranoside, has a high of degree of stability toward heat, exhibits optimal temperature activity at about 65.degree. C. at a pH range of from about 2 to about 7, has an inactivation temperature of about 80.degree. C. at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5-54.5 kD as determineded by SDS-PAGE.

Inventors:
 [1];  [2];  [3];  [2]
  1. Littleton, CO
  2. Lakewood, CO
  3. Golden, CO
Issue Date:
Research Org.:
Midwest Research Institute, Kansas City, MO (United States)
OSTI Identifier:
869971
Patent Number(s):
5432075
Assignee:
Midwest Research Institute (Kansas City, MO)
DOE Contract Number:  
AC36-83CH10093
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
molecular; weight; thermostable; beta; -d-glucosidase; acidothermus; cellulolyticus; purified; produced; atcc; 43068; enzyme; water; soluble; possesses; activity; pnp-; -d-glucopyranoside; degree; stability; heat; exhibits; optimal; temperature; 65; ph; range; inactivation; 80; 50; 5-54; kd; determineded; sds-page; ph range; cellulolyticus atcc; water soluble; molecular weight; acidothermus cellulolyticus; temperature activity; inactivation temperature; /435/

Citation Formats

Himmel, Michael E, Tucker, Melvin P, Adney, William S, and Nieves, Rafael A. Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus. United States: N. p., 1995. Web.
Himmel, Michael E, Tucker, Melvin P, Adney, William S, & Nieves, Rafael A. Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus. United States.
Himmel, Michael E, Tucker, Melvin P, Adney, William S, and Nieves, Rafael A. Sun . "Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus". United States. https://www.osti.gov/servlets/purl/869971.
@article{osti_869971,
title = {Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus},
author = {Himmel, Michael E and Tucker, Melvin P and Adney, William S and Nieves, Rafael A},
abstractNote = {A purified low molecular weight .beta.-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-.beta.-D-glucopyranoside, has a high of degree of stability toward heat, exhibits optimal temperature activity at about 65.degree. C. at a pH range of from about 2 to about 7, has an inactivation temperature of about 80.degree. C. at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5-54.5 kD as determineded by SDS-PAGE.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}

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