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Title: Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus

A purified low molecular weight .beta.-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-.beta.-D-glucopyranoside, has a high of degree of stability toward heat, exhibits optimal temperature activity at about 65.degree. C. at a pH range of from about 2 to about 7, has an inactivation temperature of about 80.degree. C. at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5-54.5 kD as determineded by SDS-PAGE.
Inventors:
 [1];  [2];  [3];  [2]
  1. (Littleton, CO)
  2. (Lakewood, CO)
  3. (Golden, CO)
Issue Date:
OSTI Identifier:
869971
Assignee:
Midwest Research Institute (Kansas City, MO) NREL
Patent Number(s):
US 5432075
Contract Number:
AC36-83CH10093
Research Org:
Midwest Research Institute
Country of Publication:
United States
Language:
English
Subject:
molecular; weight; thermostable; beta; -d-glucosidase; acidothermus; cellulolyticus; purified; produced; atcc; 43068; enzyme; water; soluble; possesses; activity; pnp-; -d-glucopyranoside; degree; stability; heat; exhibits; optimal; temperature; 65; ph; range; inactivation; 80; 50; 5-54; kd; determineded; sds-page; ph range; cellulolyticus atcc; water soluble; molecular weight; acidothermus cellulolyticus; temperature activity; inactivation temperature; /435/