Method for immunodiagnostic detection of dioxins at low concentrations

Title: Method for immunodiagnostic detection of dioxins at low concentrations

Abstract

A method is described for the use of monoclonal antibodies in a sensitive immunoassay for halogenated dioxins and dibenzofurans in industrial samples which contain impurities. Appropriate sample preparation and selective enzyme amplification of the immunoassay sensitivity permits detection of dioxin contaminants in industrial or environmental samples at concentrations in the range of a few parts per trillion.

Inventors:

Vanderlaan, Martin ^[1]; Stanker, Larry H ^[2]; Watkins, Bruce E ^[2]; Petrovic, Peter ^[3]; Gorbach, Siegbert ^[4]

Danville, CA
Livermore, CA
Hochheim am Main, DE
Eppstein/Ts., DE

Issue Date:: 1995-01-01

Research Org.:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

OSTI Identifier:: 869959

Patent Number(s):: 5429925

Assignee:: Regents of University of California (Oakland, CA)

Patent Classifications (CPCs):: Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S435/962 - Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/5308 - {for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S435/961 - including a step of forming, releasing, or exposing the antigen or forming the hapten-immunogenic carrier complex or the antigen per se
Y10S436/815 - Test for named compound or class of compounds
Y10S435/803 - Physical recovery methods, e.g. chromatography, grinding
Y10S436/825 - Pretreatment for removal of interfering factors from sample

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DOE Contract Number:: W-7405-ENG-48

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: method; immunodiagnostic; detection; dioxins; concentrations; described; monoclonal; antibodies; sensitive; immunoassay; halogenated; dibenzofurans; industrial; samples; contain; impurities; appropriate; sample; preparation; selective; enzyme; amplification; sensitivity; permits; dioxin; contaminants; environmental; range; trillion; permits detection; environmental sample; sample preparation; monoclonal antibodies; environmental samples; sensitive immunoassay; /435/436/

Citation Formats


                    Vanderlaan, Martin, Stanker, Larry H, Watkins, Bruce E, Petrovic, Peter, and Gorbach, Siegbert. Method for immunodiagnostic detection of dioxins at low concentrations.  United States: N. p., 1995. 
        Web.

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                    Vanderlaan, Martin, Stanker, Larry H, Watkins, Bruce E, Petrovic, Peter, & Gorbach, Siegbert. Method for immunodiagnostic detection of dioxins at low concentrations.  United States.

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                    Vanderlaan, Martin, Stanker, Larry H, Watkins, Bruce E, Petrovic, Peter, and Gorbach, Siegbert. Sun .  
        "Method for immunodiagnostic detection of dioxins at low concentrations".  United States.  https://www.osti.gov/servlets/purl/869959.

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@article{osti_869959,

  title        = {Method for immunodiagnostic detection of dioxins at low concentrations},

  author       = {Vanderlaan, Martin and Stanker, Larry H and Watkins, Bruce E and Petrovic, Peter and Gorbach, Siegbert},

  abstractNote = {A method is described for the use of monoclonal antibodies in a sensitive immunoassay for halogenated dioxins and dibenzofurans in industrial samples which contain impurities. Appropriate sample preparation and selective enzyme amplification of the immunoassay sensitivity permits detection of dioxin contaminants in industrial or environmental samples at concentrations in the range of a few parts per trillion.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1995},

  month        = {1}

}

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Works referenced in this record:

Monoclonal antibodies to chlorinated dibenzo-p-dioxins
journal, February 1986

Kennel, Stephen J.; Jason, Casey; Albro, Phillip W.
Toxicology and Applied Pharmacology, Vol. 82, Issue 2
https://doi.org/10.1016/0041-008X(86)90200-0

Improvement and application of an immunoassay for screening environmental samples for dioxin contamination
journal, November 1988

Vanderlaan, Martin; Stanker, Larry H.; Watkins, Bruce E.
Environmental Toxicology and Chemistry, Vol. 7, Issue 11
https://doi.org/10.1002/etc.5620071102

Analysis of polychlorinated dioxins and furans
journal, March 1984

Rappe, Christoffer
Environmental Science & Technology, Vol. 18, Issue 3
https://doi.org/10.1021/es00121a711

The use of avidin-biotin interaction in immunoenzymatic techniques.
journal, August 1979

Guesdon, J. L.; Ternynck, T.; Avrameas, S.
Journal of Histochemistry & Cytochemistry, Vol. 27, Issue 8
https://doi.org/10.1177/27.8.90074

ES&T Critical Review: Environmental monitoring by immunoassay
journal, March 1988

Vanderlaan, Martin.; Watkins, Bruce E.; Stanker, Larry.
Environmental Science & Technology, Vol. 22, Issue 3
https://doi.org/10.1021/es00168a002

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Similar Records

Title: Method for immunodiagnostic detection of dioxins at low concentrations

Abstract

Citation Formats

Monoclonal antibodies to chlorinated dibenzo-p-dioxins journal, February 1986

Improvement and application of an immunoassay for screening environmental samples for dioxin contamination journal, November 1988

Analysis of polychlorinated dioxins and furans journal, March 1984

The use of avidin-biotin interaction in immunoenzymatic techniques. journal, August 1979

ES&T Critical Review: Environmental monitoring by immunoassay journal, March 1988

Monoclonal antibodies to chlorinated dibenzo-p-dioxins
journal, February 1986

Improvement and application of an immunoassay for screening environmental samples for dioxin contamination
journal, November 1988

Analysis of polychlorinated dioxins and furans
journal, March 1984

The use of avidin-biotin interaction in immunoenzymatic techniques.
journal, August 1979

ES&T Critical Review: Environmental monitoring by immunoassay
journal, March 1988