Method for immunodiagnostic detection of dioxins at low concentrations

Title: Method for immunodiagnostic detection of dioxins at low concentrations

Full Record
Other Related Research

Abstract

A method is described for the use of monoclonal antibodies in a sensitive immunoassay for halogenated dioxins and dibenzofurans in industrial samples which contain impurities. Appropriate sample preparation and selective enzyme amplification of the immunoassay sensitivity permits detection of dioxin contaminants in industrial or environmental samples at concentrations in the range of a few parts per trillion.

Inventors:

Vanderlaan, Martin ^[1]; Stanker, Larry H ^[2]; Watkins, Bruce E ^[2]; Petrovic, Peter ^[3]; Gorbach, Siegbert ^[4]

Danville, CA
Livermore, CA
Hochheim am Main, DE
Eppstein/Ts., DE

Issue Date:: 1995-01-01

Research Org.:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

OSTI Identifier:: 869959

Patent Number(s):: 5429925

Assignee:: Regents of University of California (Oakland, CA)

DOE Contract Number:: W-7405-ENG-48

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: method; immunodiagnostic; detection; dioxins; concentrations; described; monoclonal; antibodies; sensitive; immunoassay; halogenated; dibenzofurans; industrial; samples; contain; impurities; appropriate; sample; preparation; selective; enzyme; amplification; sensitivity; permits; dioxin; contaminants; environmental; range; trillion; permits detection; environmental sample; sample preparation; monoclonal antibodies; environmental samples; sensitive immunoassay; /435/436/

Citation Formats


                    Vanderlaan, Martin, Stanker, Larry H, Watkins, Bruce E, Petrovic, Peter, and Gorbach, Siegbert. Method for immunodiagnostic detection of dioxins at low concentrations.  United States: N. p., 1995. 
        Web.

Copy to clipboard


                    Vanderlaan, Martin, Stanker, Larry H, Watkins, Bruce E, Petrovic, Peter, & Gorbach, Siegbert. Method for immunodiagnostic detection of dioxins at low concentrations.  United States.

Copy to clipboard


                    Vanderlaan, Martin, Stanker, Larry H, Watkins, Bruce E, Petrovic, Peter, and Gorbach, Siegbert. Sun .  
        "Method for immunodiagnostic detection of dioxins at low concentrations".  United States.  https://www.osti.gov/servlets/purl/869959.

Copy to clipboard


                    
@article{osti_869959,

  title        = {Method for immunodiagnostic detection of dioxins at low concentrations},

  author       = {Vanderlaan, Martin and Stanker, Larry H and Watkins, Bruce E and Petrovic, Peter and Gorbach, Siegbert},

  abstractNote = {A method is described for the use of monoclonal antibodies in a sensitive immunoassay for halogenated dioxins and dibenzofurans in industrial samples which contain impurities. Appropriate sample preparation and selective enzyme amplification of the immunoassay sensitivity permits detection of dioxin contaminants in industrial or environmental samples at concentrations in the range of a few parts per trillion.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1995},

  month        = {1}

}

Copy to clipboard

Patent:

View Patent

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE Patents and OSTI.GOV collections:

Method for detection of extremely low concentration

Patent Andresen, Brian D [Livermore, CA]; Miller, Fred S [Bethal Island, CA]

An ultratrace detector system for hand-held gas chromatography having high sensitivity, for example, to emissions generated during production of weapons, biological compounds, drugs, etc. The detector system is insensitive to water, air, helium, argon, oxygen, and CO.sub.2. The detector system is basically composed of a hand-held capillary gas chromatography (GC), an insulated heated redox-chamber, a detection chamber, and a vapor trap. For example, the detector system may use gas phase redox reactions and spectral absorption of mercury vapor. The gas chromatograph initially separates compounds that percolate through a bed of heated mercuric oxide (HgO) in a silica--or other metal--aerogel materialmore »« less
Full Text Available
Monoclonal antibodies and method for detecting dioxins and dibenzofurans

Patent Vanderlaan, Martin [San Ramon, CA]; Stanker, Larry H [Livermore, CA]; Watkins, Bruce E [Livermore, CA]; ...

Compositions of matter are described which include five monoclonal antibodies that react with dioxins and dibenzofurans, and the five hybridomas that produce these monoclonal antibodies. In addition, a method for the use of these antibodies in a sensitive immunoassay for dioxins and dibenzofurans is given, which permits detection of these pollutants in samples at concentrations in the range of a few parts per billion.
Full Text Available
Photoinduced nucleation: a novel tool for detecting molecules in air at ultra-low concentrations

Patent Katz, Joseph L [Baltimore, MD]; Lihavainen, Heikki [Masala, FI]; Rudek, Markus M [Bruchkoebel, DE]; ...

A method and apparatus for determining the presence of molecules in a gas at concentrations of less than about 100 ppb. Light having wavelengths in the range from about 200 nm to about 350 nm is used to illuminate a flowing sample of the gas causing the molecules if present to form clusters. A mixture of the illuminated gas and a vapor is cooled until the vapor is supersaturated so that there is a small rate of homogeneous nucleation. The supersaturated vapor condenses on the clusters thus causing the clusters to grow to a size sufficient to be counted bymore »« less
Full Text Available
Materials, methods and devices to detect and quantify water vapor concentrations in an atmosphere

Patent Allendorf, Mark D; Robinson, Alex L

We have demonstrated that a surface acoustic wave (SAW) sensor coated with a nanoporous framework material (NFM) film can perform ultrasensitive water vapor detection at concentrations in air from 0.05 to 12,000 ppmv at 1 atmosphere pressure. The method is extendable to other MEMS-based sensors, such as microcantilevers, or to quartz crystal microbalance sensors. We identify a specific NFM that provides high sensitivity and selectivity to water vapor. However, our approach is generalizable to detection of other species using NFM to provide sensitivity and selectivity.
Full Text Available
Method and apparatus for detecting dilute concentrations of radioactive xenon in samples of xenon extracted from the atmosphere

Patent Warburton, William K.; Hennig, Wolfgang G.

A method and apparatus for measuring the concentrations of radioxenon isotopes in a gaseous sample wherein the sample cell is surrounded by N sub-detectors that are sensitive to both electrons and to photons from radioxenon decays. Signal processing electronics are provided that can detect events within the sub-detectors, measure their energies, determine whether they arise from electrons or photons, and detect coincidences between events within the same or different sub-detectors. The energies of detected two or three event coincidences are recorded as points in associated two or three-dimensional histograms. Counts within regions of interest in the histograms are then usedmore »« less
Full Text Available

Similar Records