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Title: Ethanol production by recombinant hosts

Abstract

Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.

Inventors:
 [1];  [1];  [1];  [2];  [3];  [1];  [1]
  1. Gainesville, FL
  2. Vicosa, BR
  3. Miyazaki, JP
Issue Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
OSTI Identifier:
869925
Patent Number(s):
5424202
Assignee:
University of Florida (Gainesville, FL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG05-86ER13574
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
ethanol; production; recombinant; hosts; novel; plasmids; comprising; genes; code; alcohol; dehydrogenase; pyruvate; decarboxylase; described; transformed; coding; virtue; transformation; capable; producing; significant; amounts; fermentation; product; disclosed; methods; increasing; growth; reducing; accumulation; undesirable; metabolic; products; medium; host; oligosaccharides; encoding; polysaccharases; addition; oligomeric; feedstock; provided; method; enhancing; functional; proteins; overexpressing; adhb; process; designs; fermenting; oligosaccharide-containing; biomass; fermentation product; comprising genes; plasmids comprising; ethanol production; producing ethanol; significant amounts; alcohol dehydrogenase; recombinant host; recombinant hosts; pyruvate decarboxylase; undesirable metabolic; metabolic products; significant amount; growth medium; novel plasmids; functional proteins; genes coding; genes encoding; process design; /435/

Citation Formats

Ingram, Lonnie O, Beall, David S, Burchhardt, Gerhard F. H., Guimaraes, Walter V, Ohta, Kazuyoshi, Wood, Brent E, and Shanmugam, Keelnatham T. Ethanol production by recombinant hosts. United States: N. p., 1995. Web.
Ingram, Lonnie O, Beall, David S, Burchhardt, Gerhard F. H., Guimaraes, Walter V, Ohta, Kazuyoshi, Wood, Brent E, & Shanmugam, Keelnatham T. Ethanol production by recombinant hosts. United States.
Ingram, Lonnie O, Beall, David S, Burchhardt, Gerhard F. H., Guimaraes, Walter V, Ohta, Kazuyoshi, Wood, Brent E, and Shanmugam, Keelnatham T. Sun . "Ethanol production by recombinant hosts". United States. https://www.osti.gov/servlets/purl/869925.
@article{osti_869925,
title = {Ethanol production by recombinant hosts},
author = {Ingram, Lonnie O and Beall, David S and Burchhardt, Gerhard F. H. and Guimaraes, Walter V and Ohta, Kazuyoshi and Wood, Brent E and Shanmugam, Keelnatham T},
abstractNote = {Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}

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