Chromosome characterization using single fluorescent dye
Abstract
Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different wavelengths. A mixture containing chromosomes is stained with a single dye selected from the group consisting of TOTO and YOYO and the stained chromosomes are placed in a flow cytometer. The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or YOYO to fluoresce at a first intensity and by a second light having a wavelength effective to excite the TOTO or YOYO dye to fluoresce at a second intensity. Specific chromosomes may be identified and sorted by intensity relationships between the first and second fluorescence emissions.
- Inventors:
-
- Los Alamos, NM
- Irvine, CA
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- OSTI Identifier:
- 869894
- Patent Number(s):
- 5418169
- Assignee:
- Regents of University of California, Office of Technology (Alameda, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- chromosome; characterization; single; fluorescent; dye; chromosomes; characterized; emissions; excited; wavelengths; mixture; containing; stained; selected; consisting; toto; yoyo; placed; flow; cytometer; sequentially; light; wavelength; ultraviolet; range; excite; fluoresce; intensity; effective; specific; identified; sorted; relationships; fluorescence; mixture containing; flow cytometer; fluorescence emission; fluorescent emissions; fluorescent dye; wavelength effective; single fluorescent; fluorescence emissions; specific chromosomes; fluorescent emission; dye selected; specific chromosome; /436/209/250/435/
Citation Formats
Crissman, Harry A, and Hirons, Gregory T. Chromosome characterization using single fluorescent dye. United States: N. p., 1995.
Web.
Crissman, Harry A, & Hirons, Gregory T. Chromosome characterization using single fluorescent dye. United States.
Crissman, Harry A, and Hirons, Gregory T. Sun .
"Chromosome characterization using single fluorescent dye". United States. https://www.osti.gov/servlets/purl/869894.
@article{osti_869894,
title = {Chromosome characterization using single fluorescent dye},
author = {Crissman, Harry A and Hirons, Gregory T},
abstractNote = {Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different wavelengths. A mixture containing chromosomes is stained with a single dye selected from the group consisting of TOTO and YOYO and the stained chromosomes are placed in a flow cytometer. The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or YOYO to fluoresce at a first intensity and by a second light having a wavelength effective to excite the TOTO or YOYO dye to fluoresce at a second intensity. Specific chromosomes may be identified and sorted by intensity relationships between the first and second fluorescence emissions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}
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Works referenced in this record:
High-speed chromosome sorting
journal, October 1987
- Gray, J.; Dean, P.; Fuscoe, J.
- Science, Vol. 238, Issue 4825
Fluorometric Assay Using Dimeric Dyes for Double- and Single-Stranded DNA and RNA with Picogram Sensitivity
journal, January 1993
- Rye, H. S.; Dabora, J. M.; Quesada, M. A.
- Analytical Biochemistry, Vol. 208, Issue 1