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Title: Chromosome characterization using single fluorescent dye

Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different wavelengths. A mixture containing chromosomes is stained with a single dye selected from the group consisting of TOTO and YOYO and the stained chromosomes are placed in a flow cytometer. The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or YOYO to fluoresce at a first intensity and by a second light having a wavelength effective to excite the TOTO or YOYO dye to fluoresce at a second intensity. Specific chromosomes may be identified and sorted by intensity relationships between the first and second fluorescence emissions.
 [1];  [2]
  1. (Los Alamos, NM)
  2. (Irvine, CA)
Issue Date:
OSTI Identifier:
Regents of University of California, Office of Technology (Alameda, CA) LANL
Patent Number(s):
US 5418169
Contract Number:
Research Org:
Los Alamos National Laboratory (LANL), Los Alamos, NM
Country of Publication:
United States
chromosome; characterization; single; fluorescent; dye; chromosomes; characterized; emissions; excited; wavelengths; mixture; containing; stained; selected; consisting; toto; yoyo; placed; flow; cytometer; sequentially; light; wavelength; ultraviolet; range; excite; fluoresce; intensity; effective; specific; identified; sorted; relationships; fluorescence; mixture containing; flow cytometer; fluorescence emission; fluorescent emissions; fluorescent dye; wavelength effective; single fluorescent; fluorescence emissions; specific chromosomes; fluorescent emission; dye selected; specific chromosome; /436/209/250/435/