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Title: Chromosome characterization using single fluorescent dye

Abstract

Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different wavelengths. A mixture containing chromosomes is stained with a single dye selected from the group consisting of TOTO and YOYO and the stained chromosomes are placed in a flow cytometer. The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or YOYO to fluoresce at a first intensity and by a second light having a wavelength effective to excite the TOTO or YOYO dye to fluoresce at a second intensity. Specific chromosomes may be identified and sorted by intensity relationships between the first and second fluorescence emissions.

Inventors:
 [1];  [2]
  1. Los Alamos, NM
  2. Irvine, CA
Issue Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM
OSTI Identifier:
869894
Patent Number(s):
5418169
Assignee:
Regents of University of California, Office of Technology (Alameda, CA)
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
chromosome; characterization; single; fluorescent; dye; chromosomes; characterized; emissions; excited; wavelengths; mixture; containing; stained; selected; consisting; toto; yoyo; placed; flow; cytometer; sequentially; light; wavelength; ultraviolet; range; excite; fluoresce; intensity; effective; specific; identified; sorted; relationships; fluorescence; mixture containing; flow cytometer; fluorescence emission; fluorescent emissions; fluorescent dye; wavelength effective; single fluorescent; fluorescence emissions; specific chromosomes; fluorescent emission; dye selected; specific chromosome; /436/209/250/435/

Citation Formats

Crissman, Harry A, and Hirons, Gregory T. Chromosome characterization using single fluorescent dye. United States: N. p., 1995. Web.
Crissman, Harry A, & Hirons, Gregory T. Chromosome characterization using single fluorescent dye. United States.
Crissman, Harry A, and Hirons, Gregory T. Sun . "Chromosome characterization using single fluorescent dye". United States. https://www.osti.gov/servlets/purl/869894.
@article{osti_869894,
title = {Chromosome characterization using single fluorescent dye},
author = {Crissman, Harry A and Hirons, Gregory T},
abstractNote = {Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different wavelengths. A mixture containing chromosomes is stained with a single dye selected from the group consisting of TOTO and YOYO and the stained chromosomes are placed in a flow cytometer. The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or YOYO to fluoresce at a first intensity and by a second light having a wavelength effective to excite the TOTO or YOYO dye to fluoresce at a second intensity. Specific chromosomes may be identified and sorted by intensity relationships between the first and second fluorescence emissions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}

Patent:

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