Triple helix purification and sequencing
Abstract
Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis.
- Inventors:
-
- Dublin, CA
- Madison, WI
- Issue Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- OSTI Identifier:
- 869803
- Patent Number(s):
- 5401632
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-90ER61026
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- triple; helix; purification; sequencing; disclosed; methods; kits; equipment; purifying; single; stranded; circular; dna; purposes; templates; provided; insert; hybridization; region; elongated; oligonucleotide; regions; complementary; oligo; bound; magnetic; anchor; hybridizes; form; stable; complex; drag; template; solution; magnet; purify; desired; double; primer; synthesis; triple helix; single stranded; dna synthesis; dna sequencing; circular dna; double helix; single strand; /435/999/
Citation Formats
Wang, Renfeng, Smith, Lloyd M, and Tong, Xinchun E. Triple helix purification and sequencing. United States: N. p., 1995.
Web.
Wang, Renfeng, Smith, Lloyd M, & Tong, Xinchun E. Triple helix purification and sequencing. United States.
Wang, Renfeng, Smith, Lloyd M, and Tong, Xinchun E. Sun .
"Triple helix purification and sequencing". United States. https://www.osti.gov/servlets/purl/869803.
@article{osti_869803,
title = {Triple helix purification and sequencing},
author = {Wang, Renfeng and Smith, Lloyd M and Tong, Xinchun E},
abstractNote = {Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}
Works referenced in this record:
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