Global to push GA events into
skip to main content

Title: Method of measurement in biological systems

Disclosed is a method of quantifying molecules in biological substances comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.
Inventors:
 [1];  [2];  [3];  [4];  [1]
  1. (Livermore, CA)
  2. (Union City, CA)
  3. (Danville, CA)
  4. (Alamo, CA)
Issue Date:
OSTI Identifier:
869674
Assignee:
Regents of University of California (Oakland, CA) LLNL
Patent Number(s):
US 5376355
Contract Number:
W-7405-ENG-48
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Country of Publication:
United States
Language:
English
Subject:
method; measurement; biological; systems; disclosed; quantifying; molecules; substances; comprising; selecting; host; radioisotopes; concentrations; equal; ambient; biosphere; preparing; long-lived; radioisotope; labeled; reactive; chemical; specie; administering; doses; sufficiently; avoid; significant; overt; damage; allowing; period; time; elapse; sufficient; dissemination; interaction; throughout; isolating; reacted; fraction; substance; manner; contamination; extraneous; sources; converting; suitable; means; material; efficiently; produces; charged; introduction; isotopic; fractionation; measuring; concentration; direct; counting; reactive chemical; suitable means; avoid contamination; biological substance; biological host; biological systems; chemical specie; reacted fraction; radioisotope labeled; radioisotope concentration; significant isotopic; significant overt; avoid significant; ambient biosphere; produces charged; manner sufficient; isotopic counting; overt damage; concentrations equal; biological substances; isotopic fractionation; long-lived radioisotope; quantifying molecules; extraneous sources; direct isotopic; efficiently produces; elapse sufficient; labeled reactive; doses sufficiently; host throughout; active chemical; isotope concentration; /424/436/600/