Combined enzyme mediated fermentation of cellulous and xylose to ethanol by Schizosaccharoyces pombe, cellulase, .beta.-glucosidase, and xylose isomerase
Abstract
A process for producing ethanol from mixed sugar streams from pretreated biomass comprising xylose and cellulose using enzymes to convert these substrates to fermentable sugars; selecting and isolating a yeast Schizosaccharomyces pombe ATCC No. 2476, having the ability to ferment these sugars as they are being formed to produce ethanol; loading the substrates with the fermentation mix composed of yeast, enzymes and substrates; fermenting the loaded substrates and enzymes under anaerobic conditions at a pH range of between about 5.0 to about 6.0 and at a temperature range of between about 35.degree. C. to about 40.degree. C. until the fermentation is completed, the xylose being isomerized to xylulose, the cellulose being converted to glucose, and these sugars being concurrently converted to ethanol by yeast through means of the anaerobic fermentation; and recovering the ethanol.
- Inventors:
-
- Longmont, CO
- Northglen, CO
- Lakewood, CO
- Winter Haven, FL
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- OSTI Identifier:
- 869647
- Patent Number(s):
- 5372939
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- DOE Contract Number:
- AC02-83CH10093
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- combined; enzyme; mediated; fermentation; cellulous; xylose; ethanol; schizosaccharoyces; pombe; cellulase; beta; -glucosidase; isomerase; process; producing; mixed; sugar; streams; pretreated; biomass; comprising; cellulose; enzymes; convert; substrates; fermentable; sugars; selecting; isolating; yeast; schizosaccharomyces; atcc; 2476; ability; ferment; formed; produce; loading; mix; composed; fermenting; loaded; anaerobic; conditions; ph; range; temperature; 35; degree; 40; completed; isomerized; xylulose; converted; glucose; concurrently; means; recovering; xylose isomerase; ph range; producing ethanol; temperature range; anaerobic conditions; produce ethanol; biomass comprising; anaerobic fermentation; aerobic conditions; /435/
Citation Formats
Lastick, Stanley M, Mohagheghi, Ali, Tucker, Melvin P, and Grohmann, Karel. Combined enzyme mediated fermentation of cellulous and xylose to ethanol by Schizosaccharoyces pombe, cellulase, .beta.-glucosidase, and xylose isomerase. United States: N. p., 1994.
Web.
Lastick, Stanley M, Mohagheghi, Ali, Tucker, Melvin P, & Grohmann, Karel. Combined enzyme mediated fermentation of cellulous and xylose to ethanol by Schizosaccharoyces pombe, cellulase, .beta.-glucosidase, and xylose isomerase. United States.
Lastick, Stanley M, Mohagheghi, Ali, Tucker, Melvin P, and Grohmann, Karel. Sat .
"Combined enzyme mediated fermentation of cellulous and xylose to ethanol by Schizosaccharoyces pombe, cellulase, .beta.-glucosidase, and xylose isomerase". United States. https://www.osti.gov/servlets/purl/869647.
@article{osti_869647,
title = {Combined enzyme mediated fermentation of cellulous and xylose to ethanol by Schizosaccharoyces pombe, cellulase, .beta.-glucosidase, and xylose isomerase},
author = {Lastick, Stanley M and Mohagheghi, Ali and Tucker, Melvin P and Grohmann, Karel},
abstractNote = {A process for producing ethanol from mixed sugar streams from pretreated biomass comprising xylose and cellulose using enzymes to convert these substrates to fermentable sugars; selecting and isolating a yeast Schizosaccharomyces pombe ATCC No. 2476, having the ability to ferment these sugars as they are being formed to produce ethanol; loading the substrates with the fermentation mix composed of yeast, enzymes and substrates; fermenting the loaded substrates and enzymes under anaerobic conditions at a pH range of between about 5.0 to about 6.0 and at a temperature range of between about 35.degree. C. to about 40.degree. C. until the fermentation is completed, the xylose being isomerized to xylulose, the cellulose being converted to glucose, and these sugars being concurrently converted to ethanol by yeast through means of the anaerobic fermentation; and recovering the ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 1994},
month = {Sat Jan 01 00:00:00 EST 1994}
}
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