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Title: Combined enzyme mediated fermentation of cellulous and xylose to ethanol by Schizosaccharoyces pombe, cellulase, .beta.-glucosidase, and xylose isomerase

A process for producing ethanol from mixed sugar streams from pretreated biomass comprising xylose and cellulose using enzymes to convert these substrates to fermentable sugars; selecting and isolating a yeast Schizosaccharomyces pombe ATCC No. 2476, having the ability to ferment these sugars as they are being formed to produce ethanol; loading the substrates with the fermentation mix composed of yeast, enzymes and substrates; fermenting the loaded substrates and enzymes under anaerobic conditions at a pH range of between about 5.0 to about 6.0 and at a temperature range of between about 35.degree. C. to about 40.degree. C. until the fermentation is completed, the xylose being isomerized to xylulose, the cellulose being converted to glucose, and these sugars being concurrently converted to ethanol by yeast through means of the anaerobic fermentation; and recovering the ethanol.
Inventors:
 [1];  [2];  [3];  [4]
  1. (Longmont, CO)
  2. (Northglen, CO)
  3. (Lakewood, CO)
  4. (Winter Haven, FL)
Issue Date:
OSTI Identifier:
869647
Assignee:
United States of America as represented by United States (Washington, DC) NREL
Patent Number(s):
US 5372939
Contract Number:
AC02-83CH10093
Research Org:
MIDWEST RESEARCH INSTITUTE
Country of Publication:
United States
Language:
English
Subject:
combined; enzyme; mediated; fermentation; cellulous; xylose; ethanol; schizosaccharoyces; pombe; cellulase; beta; -glucosidase; isomerase; process; producing; mixed; sugar; streams; pretreated; biomass; comprising; cellulose; enzymes; convert; substrates; fermentable; sugars; selecting; isolating; yeast; schizosaccharomyces; atcc; 2476; ability; ferment; formed; produce; loading; mix; composed; fermenting; loaded; anaerobic; conditions; ph; range; temperature; 35; degree; 40; completed; isomerized; xylulose; converted; glucose; concurrently; means; recovering; xylose isomerase; ph range; producing ethanol; temperature range; anaerobic conditions; produce ethanol; biomass comprising; anaerobic fermentation; aerobic conditions; /435/