Derivatized gold clusters and antibody-gold cluster conjugates
Abstract
Antibody- or antibody fragment-gold cluster conjugates are shown wherein the conjugate size can be as small as 5.0 nm. Methods and reagents are disclosed in which antibodies, Fab' or F(ab').sub.2 fragments thereof are covalently bound to a stable cluster of gold atoms. The gold clusters may contain 6, 8, 9, 11, 13, 55 or 67 gold atoms in their inner core. The clusters may also contain radioactive gold. The antibody-cluster conjugates are useful in electron microscopy applications as well as in clinical applications that include imaging, diagnosis and therapy.
- Inventors:
-
- Shoreham, NY
- Williston Park, NY
- Issue Date:
- Research Org.:
- Associated Universities, Inc., Upton, NY (United States)
- OSTI Identifier:
- 869590
- Patent Number(s):
- 5360895
- Application Number:
- 07/988,338
- Assignee:
- Associated Universities, Inc. (Washington, DC)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07F - ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- derivatized; gold; clusters; antibody-gold; cluster; conjugates; antibody-; antibody; fragment-gold; shown; conjugate; size; nm; methods; reagents; disclosed; antibodies; fab; fragments; covalently; bound; stable; atoms; contain; 11; 13; 55; 67; inner; core; radioactive; antibody-cluster; useful; electron; microscopy; applications; clinical; imaging; diagnosis; therapy; inner core; covalently bound; antibody fragment; cluster conjugates; electron microscopy; /530/436/
Citation Formats
Hainfeld, James F, and Furuya, Frederic R. Derivatized gold clusters and antibody-gold cluster conjugates. United States: N. p., 1994.
Web.
Hainfeld, James F, & Furuya, Frederic R. Derivatized gold clusters and antibody-gold cluster conjugates. United States.
Hainfeld, James F, and Furuya, Frederic R. Tue .
"Derivatized gold clusters and antibody-gold cluster conjugates". United States. https://www.osti.gov/servlets/purl/869590.
@article{osti_869590,
title = {Derivatized gold clusters and antibody-gold cluster conjugates},
author = {Hainfeld, James F and Furuya, Frederic R},
abstractNote = {Antibody- or antibody fragment-gold cluster conjugates are shown wherein the conjugate size can be as small as 5.0 nm. Methods and reagents are disclosed in which antibodies, Fab' or F(ab').sub.2 fragments thereof are covalently bound to a stable cluster of gold atoms. The gold clusters may contain 6, 8, 9, 11, 13, 55 or 67 gold atoms in their inner core. The clusters may also contain radioactive gold. The antibody-cluster conjugates are useful in electron microscopy applications as well as in clinical applications that include imaging, diagnosis and therapy.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {11}
}
Works referenced in this record:
Synthesis of undecagold cluster molecules as biochemical labeling reagents. 3. Dimeric cluster with a single reactive amino group
journal, August 1984
- Yang, Heechung; Frey, Perry A.
- Biochemistry, Vol. 23, Issue 17
Synthesis of undecagold cluster molecules as biochemical labeling reagents. 2. Bromoacetyl- and maleimidoundecagold clusters
journal, August 1984
- Yang, Heechung; Reardon, John E.; Frey, Perry A.
- Biochemistry, Vol. 23, Issue 17
Californium-252 plasma desorption mass spectrometry as a tool for studying very large clusters; evidence for vertex-sharing icosahedra as components of Au67(PPh3)14Cl8
journal, August 1989
- Fackler, John P.; McNeal, C. J.; Pignolet, L. H.
- Journal of the American Chemical Society, Vol. 111, Issue 16
Collateral immunoprecipitation and localization of cellular antigens using monoclonal antibody-gold conjugates
journal, March 1983
- Goodman, S. L.; Newman, R. A.
- Experimental Cell Research, Vol. 144, Issue 1
Synthesis of undecagold cluster molecules as biochemical labeling reagents. 1. Monoacyl- and mono[N-(succinimidooxy)succinyl]undecagold clusters
journal, August 1984
- Reardon, John E.; Frey, Perry A.
- Biochemistry, Vol. 23, Issue 17
A small gold-conjugated antibody label: improved resolution for electron microscopy
journal, April 1987
- Hainfeld, J.
- Science, Vol. 236, Issue 4800
Undecagold clusters for site-specific labeling of biological macromolecules: simplified preparation and model applications
journal, February 1986
- Safer, Daniel; Bolinger, Lizann; Leigh, John S.
- Journal of Inorganic Biochemistry, Vol. 26, Issue 2
Undecagold labeling of a glycoprotein: STEM visualization of an undecagoldphosphine cluster labeling the carbohydrate sites of human haptoglobin—hemoglobin complex
journal, August 1983
- Lipka, James J.; Hainfeld, James F.; Wall, Joseph S.
- Journal of Ultrastructure Research, Vol. 84, Issue 2
Immunogold labeling of metaphase cells
journal, January 1986
- Perry, P. E.; Thomson, E. J.
- Cytogenetic and Genome Research, Vol. 41, Issue 2
Observation of an undecagold cluster compound in the scanning transmission electron microscope
journal, January 1982
- Wall, J. S.; Hainfeld, J. F.; Bartlett, P. A.
- Ultramicroscopy, Vol. 8, Issue 4
Immunospecific targeting of liposomes to cells: a novel and efficient method for covalent attachment of Fab' fragments via disulfide bonds
journal, July 1981
- Martin, Francis J.; Hubbell, Wayne L.; Papahadjopoulos, Demetrios
- Biochemistry, Vol. 20, Issue 14
Ultrastructural localization of immunoreactive neurophysins using monoclonal antibodies and protein A-gold.
journal, October 1985
- Castel, M.; Morris, J.; Ben-Barak, Y.
- Journal of Histochemistry & Cytochemistry, Vol. 33, Issue 10
Heavy Atom Cluster Labeling of Biological Specimens
journal, August 1985
- Lipka, J. J.; Hainfeld, J. F.; Wall, J. S.
- Proceedings, annual meeting, Electron Microscopy Society of America, Vol. 43
Sensitive visualization of antigen-antibody reactions in dot and blot immune overlay assays with immunogold and immunogold/silver staining
journal, November 1984
- Moeremans, M.; Daneels, G.; Van Dijck, A.
- Journal of Immunological Methods, Vol. 74, Issue 2
Gold clusters containing bidentate phosphine ligands. Preparation and X-Ray structure investigation of [Au5(dppmH)3(dppm)](NO3)2 and [Au13(dppmH)6](NO3)n
journal, January 1981
- van der Velden, J. W. A.; Vollenbroek, F. A.; Bour, J. J.
- Recueil des Travaux Chimiques des Pays-Bas, Vol. 100, Issue 4
A new class of gold cluster compounds. Synthesis and X-ray structure of the octakis(triphenylphosphinegold) dializarinsulphonate, [Au8(PPh3)8](aliz)2
journal, January 1979
- Manassero, Mario; Naldini, Luciana; Sansoni, Mirella
- Journal of the Chemical Society, Chemical Communications, Issue 9