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Title: Derivatized gold clusters and antibody-gold cluster conjugates

Abstract

Antibody- or antibody fragment-gold cluster conjugates are shown wherein the conjugate size can be as small as 5.0 nm. Methods and reagents are disclosed in which antibodies, Fab' or F(ab').sub.2 fragments thereof are covalently bound to a stable cluster of gold atoms. The gold clusters may contain 6, 8, 9, 11, 13, 55 or 67 gold atoms in their inner core. The clusters may also contain radioactive gold. The antibody-cluster conjugates are useful in electron microscopy applications as well as in clinical applications that include imaging, diagnosis and therapy.

Inventors:
 [1];  [2]
  1. Shoreham, NY
  2. Williston Park, NY
Issue Date:
Research Org.:
Associated Universities, Inc., Upton, NY (United States)
OSTI Identifier:
869590
Patent Number(s):
5360895
Application Number:
07/988,338
Assignee:
Associated Universities, Inc. (Washington, DC)
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07F - ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
derivatized; gold; clusters; antibody-gold; cluster; conjugates; antibody-; antibody; fragment-gold; shown; conjugate; size; nm; methods; reagents; disclosed; antibodies; fab; fragments; covalently; bound; stable; atoms; contain; 11; 13; 55; 67; inner; core; radioactive; antibody-cluster; useful; electron; microscopy; applications; clinical; imaging; diagnosis; therapy; inner core; covalently bound; antibody fragment; cluster conjugates; electron microscopy; /530/436/

Citation Formats

Hainfeld, James F, and Furuya, Frederic R. Derivatized gold clusters and antibody-gold cluster conjugates. United States: N. p., 1994. Web.
Hainfeld, James F, & Furuya, Frederic R. Derivatized gold clusters and antibody-gold cluster conjugates. United States.
Hainfeld, James F, and Furuya, Frederic R. Tue . "Derivatized gold clusters and antibody-gold cluster conjugates". United States. https://www.osti.gov/servlets/purl/869590.
@article{osti_869590,
title = {Derivatized gold clusters and antibody-gold cluster conjugates},
author = {Hainfeld, James F and Furuya, Frederic R},
abstractNote = {Antibody- or antibody fragment-gold cluster conjugates are shown wherein the conjugate size can be as small as 5.0 nm. Methods and reagents are disclosed in which antibodies, Fab' or F(ab').sub.2 fragments thereof are covalently bound to a stable cluster of gold atoms. The gold clusters may contain 6, 8, 9, 11, 13, 55 or 67 gold atoms in their inner core. The clusters may also contain radioactive gold. The antibody-cluster conjugates are useful in electron microscopy applications as well as in clinical applications that include imaging, diagnosis and therapy.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {11}
}

Works referenced in this record:

Gold cluster-labelled antibodies
journal, May 1988


Synthesis of undecagold cluster molecules as biochemical labeling reagents. 2. Bromoacetyl- and maleimidoundecagold clusters
journal, August 1984


Californium-252 plasma desorption mass spectrometry as a tool for studying very large clusters; evidence for vertex-sharing icosahedra as components of Au67(PPh3)14Cl8
journal, August 1989


Undecagold clusters for site-specific labeling of biological macromolecules: simplified preparation and model applications
journal, February 1986


Immunogold labeling of metaphase cells
journal, January 1986


Observation of an undecagold cluster compound in the scanning transmission electron microscope
journal, January 1982


Immunospecific targeting of liposomes to cells: a novel and efficient method for covalent attachment of Fab' fragments via disulfide bonds
journal, July 1981


Ultrastructural localization of immunoreactive neurophysins using monoclonal antibodies and protein A-gold.
journal, October 1985


Heavy Atom Cluster Labeling of Biological Specimens
journal, August 1985


Gold clusters containing bidentate phosphine ligands. Preparation and X-Ray structure investigation of [Au5(dppmH)3(dppm)](NO3)2 and [Au13(dppmH)6](NO3)n
journal, January 1981


A new class of gold cluster compounds. Synthesis and X-ray structure of the octakis(triphenylphosphinegold) dializarinsulphonate, [Au8(PPh3)8](aliz)2
journal, January 1979