Direct radiolabeling of antibody against stage specific embryonic antigen for diagnostic imaging
Abstract
Antibody against stage specific embryonic antigen-1 is radiolabeled by direct means with a radionuclide for use in detection of occult abscess and inflammation. Radiolabeling is accomplished by partial reduction of the disulfide bonds of the antibody using Sn(II), or using other reducing agents followed by the addition of Sn(II), removal of excess reducing agent and reduction by-products, and addition of a specified amount of radionuclide reducing agent, such as stannous tartrate. The resulting product may be store frozen or lyophilized, with radiolabeling accomplished by the addition of the radionuclide.
- Inventors:
-
- Albuquerque, NM
- Issue Date:
- Research Org.:
- RhoMed, Inc., Albuquerque, NM
- OSTI Identifier:
- 869490
- Patent Number(s):
- 5346687
- Assignee:
- Rhomed Incorporated (Albuquerque, NM)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- FG04-89ER60899
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- direct; radiolabeling; antibody; stage; specific; embryonic; antigen; diagnostic; imaging; antigen-1; radiolabeled; means; radionuclide; detection; occult; abscess; inflammation; accomplished; partial; reduction; disulfide; bonds; ii; reducing; agents; followed; addition; removal; excess; agent; by-products; specified; amount; stannous; tartrate; resulting; product; store; frozen; lyophilized; reducing agents; resulting product; reducing agent; specific embryonic; stage specific; diagnostic imaging; occult abscess; embryonic antigen-1; embryonic antigen; agents followed; /424/530/
Citation Formats
Rhodes, Buck A. Direct radiolabeling of antibody against stage specific embryonic antigen for diagnostic imaging. United States: N. p., 1994.
Web.
Rhodes, Buck A. Direct radiolabeling of antibody against stage specific embryonic antigen for diagnostic imaging. United States.
Rhodes, Buck A. Sat .
"Direct radiolabeling of antibody against stage specific embryonic antigen for diagnostic imaging". United States. https://www.osti.gov/servlets/purl/869490.
@article{osti_869490,
title = {Direct radiolabeling of antibody against stage specific embryonic antigen for diagnostic imaging},
author = {Rhodes, Buck A},
abstractNote = {Antibody against stage specific embryonic antigen-1 is radiolabeled by direct means with a radionuclide for use in detection of occult abscess and inflammation. Radiolabeling is accomplished by partial reduction of the disulfide bonds of the antibody using Sn(II), or using other reducing agents followed by the addition of Sn(II), removal of excess reducing agent and reduction by-products, and addition of a specified amount of radionuclide reducing agent, such as stannous tartrate. The resulting product may be store frozen or lyophilized, with radiolabeling accomplished by the addition of the radionuclide.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {1}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Murine embryonic antigen (SSEA-1) is expressed on human cells and structurally related human blood group antigen I is expressed on mouse embryos
journal, September 1982
- Knowles, Barbara B.; Rappaport, Jay; Solter, Davor
- Developmental Biology, Vol. 93, Issue 1
The labeling of high affinity sites of antibodies with 99mTc
journal, January 1985
- Paik, Chang H.; Phan, Lan N. B.; Hong, Jack J.
- International Journal of Nuclear Medicine and Biology, Vol. 12, Issue 1
Nonlymphoblastic Leukemia-Associated Antigens Identified by Monoclonal Antibodies
book, January 1985
- Andrews, Robert G.; Bernstein, Irwin D.
- Monoclonal Antibodies in Cancer
Stage-specific embryonic antigen involves αl→ 3 fucosylated type 2 blood group chains
journal, July 1981
- Gooi, H. C.; Feizi, T.; Kapadia, A.
- Nature, Vol. 292, Issue 5819
Covalent attachment of chelating groups to macromolecules
journal, July 1977
- Krejcarek, Gary E.; Tucker, Karen L.
- Biochemical and Biophysical Research Communications, Vol. 77, Issue 2
Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).
journal, November 1978
- Solter, D.; Knowles, B. B.
- Proceedings of the National Academy of Sciences, Vol. 75, Issue 11