Subtilisin variants suitable for hydrolysis and synthesis in organic media
Abstract
In accordance with the present invention, there are provided novel, modified subtilisin enzyme(s) having improved catalytic activity and/or stability in organic media.
- Inventors:
-
- Pasadena, CA
- Issue Date:
- Research Org.:
- Department of Energy (Advanced Industrial Concepts Program)
- OSTI Identifier:
- 869332
- Patent Number(s):
- 5316935
- Application Number:
- 07/864,298
- Assignee:
- California Institute of Technology (Pasadena, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- 66766
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- subtilisin; variants; suitable; hydrolysis; synthesis; organic; media; accordance; provided; novel; modified; enzyme; improved; catalytic; activity; stability; catalytic activity; organic media; improved catalytic; /435/999/
Citation Formats
Arnold, Frances H, and Chen, Keqin. Subtilisin variants suitable for hydrolysis and synthesis in organic media. United States: N. p., 1994.
Web.
Arnold, Frances H, & Chen, Keqin. Subtilisin variants suitable for hydrolysis and synthesis in organic media. United States.
Arnold, Frances H, and Chen, Keqin. Tue .
"Subtilisin variants suitable for hydrolysis and synthesis in organic media". United States. https://www.osti.gov/servlets/purl/869332.
@article{osti_869332,
title = {Subtilisin variants suitable for hydrolysis and synthesis in organic media},
author = {Arnold, Frances H and Chen, Keqin},
abstractNote = {In accordance with the present invention, there are provided novel, modified subtilisin enzyme(s) having improved catalytic activity and/or stability in organic media.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {5}
}
Works referenced in this record:
Organic solvents strip water off enzymes
journal, February 1992
- Gorman, Lu Ann S.; Dordick, Jonathan S.
- Biotechnology and Bioengineering, Vol. 39, Issue 4
Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases
journal, April 1985
- Meloun, Bedřich; Baudyš, Miroslav; Kostka, Vladimïr
- FEBS Letters, Vol. 183, Issue 2
Surface charge substitutions increase the stability of .alpha.-lytic protease in organic solvents
journal, July 1991
- Martinez, Pascal; Arnold, Frances H.
- Journal of the American Chemical Society, Vol. 113, Issue 16
Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases
journal, January 1991
- Siezen, Roland J.; de Vos, William M.; Leunissen, Jack A. M.
- "Protein Engineering, Design and Selection", Vol. 4, Issue 7
Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering
journal, February 1987
- Russell, Alan J.; Thomas, Paul G.; Fersht, Alan R.
- Journal of Molecular Biology, Vol. 193, Issue 4
The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation.
journal, June 1988
- Bott, R.; Ultsch, M.; Kossiakoff, A.
- Journal of Biological Chemistry, Vol. 263, Issue 16
Effects of pH and urea on the conformational properties of subtilisin DY
journal, November 1982
- Ricchelli, F.; Jori, G.; Filippi, B.
- Biochemical Journal, Vol. 207, Issue 2
Enzyme function in organic solvents
journal, January 1992
- Gupta, Munishwar N.
- European Journal of Biochemistry, Vol. 203, Issue 1-2
Engineering enzymes for non-aqueous solvents
journal, January 1990
- Arnold, F.
- Trends in Biotechnology, Vol. 8
Enzyme engineering for nonaqueous solvents. II. Additive effects of mutations on the stability and activity of subtilisin E in polar organic media
journal, March 1991
- Chen, Keqin; Robinson, Amy C.; Van Dam, Mariana E.
- Biotechnology Progress, Vol. 7, Issue 2
Enzyme Engineering for Nonaqueous Solvents: Random Mutagenesis to Enhance Activity of Subtilisin E in Polar Organic Media
journal, November 1991
- Chen, Keqin; Arnold, Frances H.
- Bio/Technology, Vol. 9, Issue 11
Hydrophilicity of polar amino acid side-chains is markedly reduced by flanking peptide bonds
journal, April 1988
- Roseman, Mark A.
- Journal of Molecular Biology, Vol. 200, Issue 3
Stabilization of substilisin E in organic solvents by site-directed mutagenesis
journal, January 1992
- Martinez, Pascal; Van Dam, Mariana E.; Robinson, Amy C.
- Biotechnology and Bioengineering, Vol. 39, Issue 2
Random mutagenesis to enhance the activity of subtilisin in organic solvents: Characterization of Q103R subtilisin E
journal, March 1992
- Economou, Chariklia; Chen, Keqin; Arnold, Frances H.
- Biotechnology and Bioengineering, Vol. 39, Issue 6
Enzymes in organic synthesis: use of subtilisin and a highly stable mutant derived from multiple site-specific mutations
journal, January 1990
- Wong, Chi Huey; Chen, S. T.; Hennen, William J.
- Journal of the American Chemical Society, Vol. 112, Issue 3
Engineering subtilisin for reaction in dimethylformamide
journal, January 1991
- Zhong, Ziyang; Liu, Jennifer L. C.; Dinterman, Lois M.
- Journal of the American Chemical Society, Vol. 113, Issue 2