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Title: Subtilisin variants suitable for hydrolysis and synthesis in organic media

Abstract

In accordance with the present invention, there are provided novel, modified subtilisin enzyme(s) having improved catalytic activity and/or stability in organic media.

Inventors:
 [1];  [1]
  1. Pasadena, CA
Issue Date:
Research Org.:
Department of Energy (Advanced Industrial Concepts Program)
OSTI Identifier:
869332
Patent Number(s):
5316935
Application Number:
07/864,298
Assignee:
California Institute of Technology (Pasadena, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
66766
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
subtilisin; variants; suitable; hydrolysis; synthesis; organic; media; accordance; provided; novel; modified; enzyme; improved; catalytic; activity; stability; catalytic activity; organic media; improved catalytic; /435/999/

Citation Formats

Arnold, Frances H, and Chen, Keqin. Subtilisin variants suitable for hydrolysis and synthesis in organic media. United States: N. p., 1994. Web.
Arnold, Frances H, & Chen, Keqin. Subtilisin variants suitable for hydrolysis and synthesis in organic media. United States.
Arnold, Frances H, and Chen, Keqin. Tue . "Subtilisin variants suitable for hydrolysis and synthesis in organic media". United States. https://www.osti.gov/servlets/purl/869332.
@article{osti_869332,
title = {Subtilisin variants suitable for hydrolysis and synthesis in organic media},
author = {Arnold, Frances H and Chen, Keqin},
abstractNote = {In accordance with the present invention, there are provided novel, modified subtilisin enzyme(s) having improved catalytic activity and/or stability in organic media.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {5}
}

Works referenced in this record:

Organic solvents strip water off enzymes
journal, February 1992


Surface charge substitutions increase the stability of .alpha.-lytic protease in organic solvents
journal, July 1991


Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases
journal, January 1991


Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering
journal, February 1987


Effects of pH and urea on the conformational properties of subtilisin DY
journal, November 1982


Enzyme function in organic solvents
journal, January 1992


Engineering enzymes for non-aqueous solvents
journal, January 1990


Stabilization of substilisin E in organic solvents by site-directed mutagenesis
journal, January 1992


Random mutagenesis to enhance the activity of subtilisin in organic solvents: Characterization of Q103R subtilisin E
journal, March 1992


Enzymes in organic synthesis: use of subtilisin and a highly stable mutant derived from multiple site-specific mutations
journal, January 1990


Engineering subtilisin for reaction in dimethylformamide
journal, January 1991