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Title: Dyes designed for high sensitivity detection of double-stranded DNA

Abstract

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

Inventors:
 [1];  [2]
  1. Orinda, CA
  2. Albany, CA
Issue Date:
Research Org.:
Univ. of California (United States)
OSTI Identifier:
869304
Patent Number(s):
5312921
Assignee:
Regents of University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07D - HETEROCYCLIC COMPOUNDS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FG03-91ER61125
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
dyes; designed; sensitivity; detection; double-stranded; dna; novel; fluorescent; provided; characterized; fluorophore; joined; polycationic; chain; positive; charges; found; provide; enhancement; binding; nucleic; acid; strong; affinities; compared; dsdna; electrophoresis; solution; substantially; sensitivities; dye; novel fluorescent; stranded dna; dyes designed; double-stranded dna; nucleic acid; fluorescent dyes; fluorescent dye; sensitivity detection; strong binding; binding affinities; polycationic chain; cationic chain; fluorophore joined; /999/

Citation Formats

Glazer, Alexander N, and Benson, Scott C. Dyes designed for high sensitivity detection of double-stranded DNA. United States: N. p., 1994. Web.
Glazer, Alexander N, & Benson, Scott C. Dyes designed for high sensitivity detection of double-stranded DNA. United States.
Glazer, Alexander N, and Benson, Scott C. Sat . "Dyes designed for high sensitivity detection of double-stranded DNA". United States. https://www.osti.gov/servlets/purl/869304.
@article{osti_869304,
title = {Dyes designed for high sensitivity detection of double-stranded DNA},
author = {Glazer, Alexander N and Benson, Scott C},
abstractNote = {Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {1}
}

Patent:

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Works referenced in this record:

Color and Constitution. V. 1 The Absorption of Unsymmetrical Cyanines. Resonance as a Basis for a Classification of Dyes
journal, February 1942


Excitation energy dependence of intramolecular excimer formation in N,N,N',N'-tetramethylpropanediamine vapor
journal, February 1983


Color and Constitution. II. 1 Absorptions of Some Related Vinylene-Homologous Series *
journal, November 1941


Thiazole orange: A new dye for reticulocyte analysis
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Capillary array electrophoresis: an approach to high-speed, high-throughput DNA sequencing
journal, September 1992


Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications
journal, January 1992


Capillary electrophoresis with laser-induced fluorescence detection of PCR fragments using thiazole orange
journal, August 1992