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Title: Dyes designed for high sensitivity detection of double-stranded DNA

Abstract

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

Inventors:
 [1];  [2]
  1. (Orinda, CA)
  2. (Albany, CA)
Issue Date:
Research Org.:
University of California
OSTI Identifier:
869304
Patent Number(s):
5312921
Assignee:
Regents of University of California (Oakland, CA) OAK
DOE Contract Number:  
FG03-91ER61125
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
dyes; designed; sensitivity; detection; double-stranded; dna; novel; fluorescent; provided; characterized; fluorophore; joined; polycationic; chain; positive; charges; found; provide; enhancement; binding; nucleic; acid; strong; affinities; compared; dsdna; electrophoresis; solution; substantially; sensitivities; dye; novel fluorescent; stranded dna; dyes designed; double-stranded dna; nucleic acid; fluorescent dyes; fluorescent dye; sensitivity detection; strong binding; binding affinities; polycationic chain; cationic chain; fluorophore joined; /999/

Citation Formats

Glazer, Alexander N., and Benson, Scott C. Dyes designed for high sensitivity detection of double-stranded DNA. United States: N. p., 1994. Web.
Glazer, Alexander N., & Benson, Scott C. Dyes designed for high sensitivity detection of double-stranded DNA. United States.
Glazer, Alexander N., and Benson, Scott C. Sat . "Dyes designed for high sensitivity detection of double-stranded DNA". United States. https://www.osti.gov/servlets/purl/869304.
@article{osti_869304,
title = {Dyes designed for high sensitivity detection of double-stranded DNA},
author = {Glazer, Alexander N. and Benson, Scott C.},
abstractNote = {Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {1}
}

Patent:

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