skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Catalytic and reactive polypeptides and methods for their preparation and use

Abstract

Catalytic and reactive polypeptides include a binding site specific for a reactant or reactive intermediate involved in a chemical reaction of interest. The polypeptides further include at least one active functionality proximate the binding site, where the active functionality is capable of catalyzing or chemically participating in the chemical reaction in such a way that the reaction rate is enhanced. Methods for preparing the catalytic peptides include chemical synthesis, site-directed mutagenesis of antibody and enzyme genes, covalent attachment of the functionalities through particular amino acid side chains, and the like.

Inventors:
 [1]
  1. Oakland, CA
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
OSTI Identifier:
869234
Patent Number(s):
5302516
Assignee:
Regents of University of California (Berkeley, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
DOE Contract Number:  
C87-101226
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
catalytic; reactive; polypeptides; methods; preparation; binding; site; specific; reactant; intermediate; involved; chemical; reaction; active; functionality; proximate; capable; catalyzing; chemically; participating; rate; enhanced; preparing; peptides; synthesis; site-directed; mutagenesis; antibody; enzyme; genes; covalent; attachment; functionalities; particular; amino; acid; chains; reactive polypeptides; chemical synthesis; active functionality; reaction rate; binding site; amino acid; chemical reaction; reactive intermediate; site specific; functionality proximate; intermediate involved; active poly; active polypeptide; /435/

Citation Formats

Schultz, Peter. Catalytic and reactive polypeptides and methods for their preparation and use. United States: N. p., 1994. Web.
Schultz, Peter. Catalytic and reactive polypeptides and methods for their preparation and use. United States.
Schultz, Peter. Sat . "Catalytic and reactive polypeptides and methods for their preparation and use". United States. https://www.osti.gov/servlets/purl/869234.
@article{osti_869234,
title = {Catalytic and reactive polypeptides and methods for their preparation and use},
author = {Schultz, Peter},
abstractNote = {Catalytic and reactive polypeptides include a binding site specific for a reactant or reactive intermediate involved in a chemical reaction of interest. The polypeptides further include at least one active functionality proximate the binding site, where the active functionality is capable of catalyzing or chemically participating in the chemical reaction in such a way that the reaction rate is enhanced. Methods for preparing the catalytic peptides include chemical synthesis, site-directed mutagenesis of antibody and enzyme genes, covalent attachment of the functionalities through particular amino acid side chains, and the like.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {1}
}

Patent:

Save / Share:

Works referenced in this record:

Antibody Catalysis by Transition State Stabilization
journal, January 1987


Designer catalytic antibodies
journal, March 1989


A semisynthetic catalytic antibody
journal, March 1989


Catalysis of concerted reactions by antibodies: the Claisen rearrangement.
journal, July 1988


Transaminations by pyridoxamine selectively attached at C-3 in .beta.-cyclodextrin
journal, March 1983


Principles of antibody catalysis
journal, October 1988


Engineering antibody affinity and specificity
journal, January 1988


A-(modified B6)-B-[.omega.-amino(ethylamino)]-.beta.-cyclodextrin as an artificial B6 enzyme for chiral aminotransfer reaction
journal, September 1985


Intramolecular general base-acid catalysis in transaminations catalyzed by pyridoxamine enzyme analogs
journal, March 1983


Induction of an antibody that catalyzes the hydrolysis of an amide bond
journal, September 1988


Catalytic antibodies
journal, April 1987


Host-guest complexation. 31. A transacylase partial mimic
journal, August 1984


Catalysis and chiral recognition through designed complexation of transition states in transacylations of amino ester salts
journal, February 1976


Molecular catalysis: enhanced rates of thiolysis with high structural and chiral recognition in complexes of a reactive macrocyclic receptor molecule
journal, January 1978


Artificial enzymes
journal, November 1982


Reversible oxygenation and autoxidation of a capped porphyrin iron(II) complex
journal, January 1975


Antibody catalysis approaching the activity of enzymes
journal, March 1988


Generation of a hybrid sequence-specific single-stranded deoxyribonuclease
journal, December 1987


A stereospecific cyclization catalyzed by an antibody
journal, August 1987


The conversion of serine at the active site of subtilisin to cysteine: a "chemical mutation".
journal, November 1966


Catalysis of a stereospecific bimolecular amide synthesis by an antibody.
journal, August 1988


Cyclodextrin catalysis as a model for enzyme action
journal, March 1982


Antibody catalysis of bimolecular amide formation
journal, July 1988


Chemical mutation of enzyme active sites
journal, November 1984


Photosensitized cleavage of a thymine dimer by an antibody
journal, November 1988


Antibodies as enzymes
journal, January 1987


A new strategy for the generation of catalytic antibodies
journal, March 1989


An antibody-catalyzed Claisen rearrangement
journal, July 1988


Synthesis of the Myoglobin Active Site
journal, September 1973


Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.
journal, November 1982


Synthetic models for the oxygen-binding hemoproteins
journal, July 1977


Catalytic antibodies
journal, December 1986


Directed mutagenesis of the redox-active disulfide in the flavoenzyme mercuric ion reductase
journal, November 1985


Selective chemical catalysis by an antibody
journal, December 1986


Introduction of nucleophiles and spectroscopic probes into antibody combining sites
journal, November 1988


Redesigning trypsin: alteration of substrate specificity
journal, April 1985


A large increase in enzyme–substrate affinity by protein engineering
journal, January 1984


Linkage of cytotoxic agents to immunoglobulins
journal, January 1983


A New Enzyme Containing a Synthetically Formed Active Site. Thiol-Subtilisin 1
journal, July 1966


Chemical reactivity at an antibody binding site elicited by mechanistic design of a synthetic antigen.
journal, September 1986


Eine über Antikörper gesteuerte Redoxreaktion
journal, September 1988