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Title: Self-referencing spectrophotometric measurements

A method for measuring the concentration of a chemical substance by spectrophotometry comprising the steps of placing a sample of a photoreactive substance between the light source and a spectrophotometer, obtaining an absorption spectrum of the substance using a fixed amount of light from the light source, obtaining a second absorption spectrum after a short interval, comparing the two to determine the concentration of the chemical substance from the difference in the spectra. If the chemical substance is not photoreactive, a photoreactive mixture can be made with a photoreactive dye that has photoreactive properties unique to the mixture. Alternatively, an optically transparent substrate can absorb the substance or the dye/substance mixture.
Inventors:
 [1];  [2]
  1. (Martinez, GA)
  2. (Aiken, SC)
Issue Date:
OSTI Identifier:
869214
Assignee:
United States Department of Energy (Washington, DC) SRS
Patent Number(s):
US 5298428
Contract Number:
AC09-76SR00001
Research Org:
E I DUPONT DE NEMOURS & CO INC
Country of Publication:
United States
Language:
English
Subject:
self-referencing; spectrophotometric; measurements; method; measuring; concentration; chemical; substance; spectrophotometry; comprising; steps; placing; sample; photoreactive; light; source; spectrophotometer; obtaining; absorption; spectrum; fixed; amount; interval; comparing; determine; difference; spectra; mixture; dye; properties; unique; alternatively; optically; transparent; substrate; absorb; transparent substrate; spectrophotometric measurements; optically transparent; light source; absorption spectrum; chemical substance; parent substrate; active substance; /436/356/422/