skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068

Abstract

A purified low molecular weight cellulase endoglucanase I having a molecular weight of between about 57,420 to about 74,580 daltons from Acidothermus cellulolyticus (ATCC 43068). The cellulase is water soluble, possesses both C.sub.1 and C.sub.x types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 83.degree. C. at pH's from about 2 to about 9, and in inactivation temperature of about 110.degree. C. at pH's from about 2 to about 9.

Inventors:
 [1];  [2];  [3];  [4]
  1. Littleton, CO
  2. Golden, CO
  3. Lakewood, CO
  4. Winter Haven, FL
Issue Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States); Midwest Research Institute, Kansas City, MO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
869113
Patent Number(s):
5275944
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC02-83CH10093
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
thermostable; purified; endoglucanas; acidothermus; cellulolyticus; atcc; 43068; molecular; weight; cellulase; endoglucanase; 57; 420; 74; 580; daltons; water; soluble; possesses; types; enzyme; activity; degree; stability; heat; exhibits; optimum; temperature; 83; ph; inactivation; 110; cellulolyticus atcc; optimum temperature; enzyme activity; water soluble; molecular weight; acidothermus cellulolyticus; thermostable purified; temperature activity; weight cellulase; inactivation temperature; purified endoglucanas; /435/

Citation Formats

Himmel, Michael E, Adney, William S, Tucker, Melvin P, and Grohmann, Karel. Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068. United States: N. p., 1994. Web.
Himmel, Michael E, Adney, William S, Tucker, Melvin P, & Grohmann, Karel. Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068. United States.
Himmel, Michael E, Adney, William S, Tucker, Melvin P, and Grohmann, Karel. Sat . "Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068". United States. https://www.osti.gov/servlets/purl/869113.
@article{osti_869113,
title = {Thermostable purified endoglucanas from acidothermus cellulolyticus ATCC 43068},
author = {Himmel, Michael E and Adney, William S and Tucker, Melvin P and Grohmann, Karel},
abstractNote = {A purified low molecular weight cellulase endoglucanase I having a molecular weight of between about 57,420 to about 74,580 daltons from Acidothermus cellulolyticus (ATCC 43068). The cellulase is water soluble, possesses both C.sub.1 and C.sub.x types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 83.degree. C. at pH's from about 2 to about 9, and in inactivation temperature of about 110.degree. C. at pH's from about 2 to about 9.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {1}
}

Patent:

Save / Share:

Works referenced in this record:

Bacterium produces thermostable cellulase
journal, May 1987


Ultra-Thermostable Cellulases From Acidothermus cellulolyticus: Comparison of Temperature Optima with Previously Reported Cellulases
journal, August 1989


Cellulolytic enzyme system of Acetivibrio cellulolyticus
journal, March 1981


Mechanisms of Thermophily
journal, January 1978


Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria
journal, July 1986