Method for sequencing DNA base pairs

Sessler, Andrew M; Dawson, John

Title: Method for sequencing DNA base pairs

Abstract

The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source.

Inventors:

Sessler, Andrew M ^[1]; Dawson, John ^[2]

Oakland, CA
Pacific Palisades, CA

Issue Date:: 1993-01-01

Research Org.:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

OSTI Identifier:: 869068

Patent Number(s):: 5270214

Assignee:: United States of America as represented by United States (Washington, DC)

Patent Classifications (CPCs):: Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

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Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S977/853 - Biological sample

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
Y10T436/143333 - Saccharide [e.g., DNA, etc.]

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/6869 - Methods for sequencing

G - PHYSICS G01 - MEASURING G01Q - SCANNING-PROBE TECHNIQUES OR APPARATUS
G01Q30/10 - Thermal environment

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q2565/601 - being a microscope, e.g. atomic force microscopy [AFM]

G - PHYSICS G01 - MEASURING G01Q - SCANNING-PROBE TECHNIQUES OR APPARATUS
G01Q30/02 - Non-SPM analysing devices, e.g. SEM [Scanning Electron Microscope], spectrometer or optical microscope

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/48 - Biological material, e.g. blood, urine

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
B82Y35/00 - Methods or apparatus for measurement or analysis of nanostructures

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DOE Contract Number:: AC03-76SF00098

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: method; sequencing; dna; base; pairs; structure; sequenced; scanning; tunneling; microscope; stm; scanned; probe; tip; separately; subjected; sequence; infrared; radiation; sources; source; selected; preferentially; excite; bases; particular; imaged; subjection; scanning tunneling; sequencing dna; tunneling microscope; probe tip; infrared radiation; dna structure; base pairs; base pair; preferentially excite; /436/250/

Citation Formats


                    Sessler, Andrew M, and Dawson, John. Method for sequencing DNA base pairs.  United States: N. p., 1993. 
        Web.

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                    Sessler, Andrew M, & Dawson, John. Method for sequencing DNA base pairs.  United States.

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                    Sessler, Andrew M, and Dawson, John. Fri .  
        "Method for sequencing DNA base pairs".  United States.  https://www.osti.gov/servlets/purl/869068.

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@article{osti_869068,

  title        = {Method for sequencing DNA base pairs},

  author       = {Sessler, Andrew M and Dawson, John},

  abstractNote = {The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1993},

  month        = {1}

}

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Works referenced in this record:

Direct observation of native DNA structures with the scanning tunneling microscope
journal, January 1989

Beebe, T.; Wilson, T.; Ogletree, D.
Science, Vol. 243, Issue 4889
https://doi.org/10.1126/science.2911747

Imaging of single uncoated DNA molecules by scanning tunneling microscopy.
journal, July 1989

Keller, D.; Bustamante, C.; Keller, R. W.
Proceedings of the National Academy of Sciences, Vol. 86, Issue 14
https://doi.org/10.1073/pnas.86.14.5356

Images of single-stranded nucleic acids by scanning tunnelling microscopy
journal, November 1989

Dunlap, David D.; Bustamante, Carlos
Nature, Vol. 342, Issue 6246
https://doi.org/10.1038/342204a0

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Title: Method for sequencing DNA base pairs

Abstract

Citation Formats

Direct observation of native DNA structures with the scanning tunneling microscope journal, January 1989

Imaging of single uncoated DNA molecules by scanning tunneling microscopy. journal, July 1989

Images of single-stranded nucleic acids by scanning tunnelling microscopy journal, November 1989

Direct observation of native DNA structures with the scanning tunneling microscope
journal, January 1989

Imaging of single uncoated DNA molecules by scanning tunneling microscopy.
journal, July 1989

Images of single-stranded nucleic acids by scanning tunnelling microscopy
journal, November 1989