Method for sequencing DNA base pairs
Abstract
The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source.
- Inventors:
-
- Oakland, CA
- Pacific Palisades, CA
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- OSTI Identifier:
- 869068
- Patent Number(s):
- 5270214
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Classifications (CPCs):
-
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; sequencing; dna; base; pairs; structure; sequenced; scanning; tunneling; microscope; stm; scanned; probe; tip; separately; subjected; sequence; infrared; radiation; sources; source; selected; preferentially; excite; bases; particular; imaged; subjection; scanning tunneling; sequencing dna; tunneling microscope; probe tip; infrared radiation; dna structure; base pairs; base pair; preferentially excite; /436/250/
Citation Formats
Sessler, Andrew M, and Dawson, John. Method for sequencing DNA base pairs. United States: N. p., 1993.
Web.
Sessler, Andrew M, & Dawson, John. Method for sequencing DNA base pairs. United States.
Sessler, Andrew M, and Dawson, John. Fri .
"Method for sequencing DNA base pairs". United States. https://www.osti.gov/servlets/purl/869068.
@article{osti_869068,
title = {Method for sequencing DNA base pairs},
author = {Sessler, Andrew M and Dawson, John},
abstractNote = {The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {1}
}
Works referenced in this record:
Direct observation of native DNA structures with the scanning tunneling microscope
journal, January 1989
- Beebe, T.; Wilson, T.; Ogletree, D.
- Science, Vol. 243, Issue 4889
Imaging of single uncoated DNA molecules by scanning tunneling microscopy.
journal, July 1989
- Keller, D.; Bustamante, C.; Keller, R. W.
- Proceedings of the National Academy of Sciences, Vol. 86, Issue 14
Images of single-stranded nucleic acids by scanning tunnelling microscopy
journal, November 1989
- Dunlap, David D.; Bustamante, Carlos
- Nature, Vol. 342, Issue 6246