Title: Gene encoding plant asparagine synthetase

Abstract

The identification and cloning of the gene(s) for plant asparagine synthetase (AS), an important enzyme involved in the formation of asparagine, a major nitrogen transport compound of higher plants is described. Expression vectors constructed with the AS coding sequence may be utilized to produce plant AS; to engineer herbicide resistant plants, salt/drought tolerant plants or pathogen resistant plants; as a dominant selectable marker; or to select for novel herbicides or compounds useful as agents that synchronize plant cells in culture. The promoter for plant AS, which directs high levels of gene expression and is induced in an organ specific manner and by darkness, is also described. The AS promoter may be used to direct the expression of heterologous coding sequences in appropriate hosts.

Inventors:

Coruzzi, Gloria M ^[1]; Tsai, Fong-Ying ^[1]

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1. New York, NY

Issue Date:

1993-10-26

OSTI Identifier:

868982

Patent Number(s):

5256558

Application Number:

07/514,816

Assignee:

Trustees of Rockefeller University (New York, NY)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K16/40 - against enzymes

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/8209 - {Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers}
C12N15/8222 - {Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation}
C12N15/8227 - {Root-specific}
C12N15/8237 - {Externally regulated expression systems}
C12N15/8273 - {for drought, cold, salt resistance}
C12N9/93 - {Ligases

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Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

encoding; plant; asparagine; synthetase; identification; cloning; enzyme; involved; formation; major; nitrogen; transport; compound; plants; described; expression; vectors; constructed; coding; sequence; utilized; produce; engineer; herbicide; resistant; salt; drought; tolerant; pathogen; dominant; selectable; marker; select; novel; herbicides; compounds; useful; agents; synchronize; cells; culture; promoter; directs; levels; induced; organ; specific; manner; darkness; direct; heterologous; sequences; appropriate; hosts; compounds useful; coding sequences; plant cell; plant cells; resistant plants; coding sequence; expression vectors; expression vector; specific manner; plant asparagine; asparagine synthetase; /435/999/