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Title: Certain tricyclic and pentacyclic-hetero nitrogen rhodol dyes

Abstract

Novel fluorescent dyes based on the rhodol structure are provided. The new reagents contain functional groups capable of forming a stable fluorescent product with functional groups typically found in biomolecules or polymers including amines, phenols, thiols, acids, aldehydes and ketones. Reactive groups in the rhodol dyes include activated esters, isothiocyanates, amines, hydrazines, halides, acids, azides, maleimides, aldehydes, alcohols, acrylamides and haloacetamides. The products are detected by their absorbance or fluorescence properties. The spectral properties of the fluorescent dyes are sufficiently similar in wavelengths and intensity to fluorescein or rhodamine derivatives as to permit use of the same equipment. The dyes, however, show less spectral sensitivity to pH in the physiological range than does fluorescein, have higher solubility in non-polar solvents and have improved photostability and quantum yields.

Inventors:
 [1];  [1]
  1. (Eugene, OR)
Issue Date:
Research Org.:
Molecular Probes, Inc. (Eugene, OR)
OSTI Identifier:
868857
Patent Number(s):
5227487
Assignee:
Molecular Probes, Inc. (Eugene, OR) RLO
DOE Contract Number:  
FG06-88ER60684
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
tricyclic; pentacyclic-hetero; nitrogen; rhodol; dyes; novel; fluorescent; based; structure; provided; reagents; contain; functional; capable; forming; stable; product; typically; found; biomolecules; polymers; including; amines; phenols; thiols; acids; aldehydes; ketones; reactive; activated; esters; isothiocyanates; hydrazines; halides; azides; maleimides; alcohols; acrylamides; haloacetamides; products; detected; absorbance; fluorescence; properties; spectral; sufficiently; similar; wavelengths; intensity; fluorescein; rhodamine; derivatives; permit; equipment; sensitivity; ph; physiological; range; solubility; non-polar; solvents; improved; photostability; quantum; yields; novel fluorescent; polar solvent; fluorescent dyes; polymers including; fluorescent dye; spectral properties; fluorescent product; improved photostability; reagents contain; polar solvents; /999/436/530/

Citation Formats

Haugland, Richard P., and Whitaker, James E. Certain tricyclic and pentacyclic-hetero nitrogen rhodol dyes. United States: N. p., 1993. Web.
Haugland, Richard P., & Whitaker, James E. Certain tricyclic and pentacyclic-hetero nitrogen rhodol dyes. United States.
Haugland, Richard P., and Whitaker, James E. Fri . "Certain tricyclic and pentacyclic-hetero nitrogen rhodol dyes". United States. https://www.osti.gov/servlets/purl/868857.
@article{osti_868857,
title = {Certain tricyclic and pentacyclic-hetero nitrogen rhodol dyes},
author = {Haugland, Richard P. and Whitaker, James E.},
abstractNote = {Novel fluorescent dyes based on the rhodol structure are provided. The new reagents contain functional groups capable of forming a stable fluorescent product with functional groups typically found in biomolecules or polymers including amines, phenols, thiols, acids, aldehydes and ketones. Reactive groups in the rhodol dyes include activated esters, isothiocyanates, amines, hydrazines, halides, acids, azides, maleimides, aldehydes, alcohols, acrylamides and haloacetamides. The products are detected by their absorbance or fluorescence properties. The spectral properties of the fluorescent dyes are sufficiently similar in wavelengths and intensity to fluorescein or rhodamine derivatives as to permit use of the same equipment. The dyes, however, show less spectral sensitivity to pH in the physiological range than does fluorescein, have higher solubility in non-polar solvents and have improved photostability and quantum yields.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {1}
}

Patent:

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