Method of measurement in biological systems
Abstract
Disclosed is a method of quantifying molecules in biological substances, comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.
- Inventors:
-
- Livermore, CA
- Union City, CA
- Danville, CA
- Alamo, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 868782
- Patent Number(s):
- 5209919
- Application Number:
- 07/693,248
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; measurement; biological; systems; disclosed; quantifying; molecules; substances; comprising; selecting; host; radioisotopes; concentrations; equal; ambient; biosphere; preparing; long-lived; radioisotope; labeled; reactive; chemical; specie; administering; doses; sufficiently; avoid; significant; overt; damage; allowing; period; time; elapse; sufficient; dissemination; interaction; throughout; isolating; reacted; fraction; substance; manner; contamination; extraneous; sources; converting; suitable; means; material; efficiently; produces; charged; introduction; isotopic; fractionation; measuring; concentration; direct; counting; reactive chemical; suitable means; avoid contamination; biological substance; biological host; biological systems; chemical specie; reacted fraction; radioisotope labeled; radioisotope concentration; significant isotopic; significant overt; avoid significant; ambient biosphere; produces charged; manner sufficient; isotopic counting; overt damage; concentrations equal; biological substances; isotopic fractionation; long-lived radioisotope; quantifying molecules; extraneous sources; direct isotopic; efficiently produces; elapse sufficient; labeled reactive; doses sufficiently; host throughout; active chemical; isotope concentration; /424/250/436/600/
Citation Formats
Turteltaub, Kenneth W, Vogel, John S, Felton, James S, Gledhill, Barton L, Davis, Jay C, and Stanker, Larry H. Method of measurement in biological systems. United States: N. p., 1993.
Web.
Turteltaub, Kenneth W, Vogel, John S, Felton, James S, Gledhill, Barton L, Davis, Jay C, & Stanker, Larry H. Method of measurement in biological systems. United States.
Turteltaub, Kenneth W, Vogel, John S, Felton, James S, Gledhill, Barton L, Davis, Jay C, and Stanker, Larry H. Tue .
"Method of measurement in biological systems". United States. https://www.osti.gov/servlets/purl/868782.
@article{osti_868782,
title = {Method of measurement in biological systems},
author = {Turteltaub, Kenneth W and Vogel, John S and Felton, James S and Gledhill, Barton L and Davis, Jay C and Stanker, Larry H},
abstractNote = {Disclosed is a method of quantifying molecules in biological substances, comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 11 00:00:00 EDT 1993},
month = {Tue May 11 00:00:00 EDT 1993}
}
Works referenced in this record:
Accelerator mass spectrometry in biomedical dosimetry: relationship between low-level exposure and covalent binding of heterocyclic amine carcinogens to DNA.
journal, July 1990
- Turteltaub, K. W.; Felton, J. S.; Gledhill, B. L.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 14
Accelerator mass spectrometry in the biomedical sciences: Applications in low-exposure biomedical and environmental dosimetry
report, May 1990
- Felton, J.; Turteltaub, K.; Vogel, J.
Ultrasensitive radioisotope, stable-isotope, and trace-element analysis in the biological sciences using tandem accelerator mass spectrometry
journal, April 1987
- Elmore, David
- Biological Trace Element Research, Vol. 12, Issue 1
Ultrasensitive Mass Spectrometry with Accelerators
journal, December 1980
- Litherland, A. E.
- Annual Review of Nuclear and Particle Science, Vol. 30, Issue 1
Applications of Accelerator Mass Spectrometry
journal, May 1984
- Brown, L.
- Annual Review of Earth and Planetary Sciences, Vol. 12, Issue 1
Memory Effects in an AMS System: Catastrophe and Recovery
journal, January 1990
- Vogel, J. S.; Southon, J. R.; Nelson, D. E.
- Radiocarbon, Vol. 32, Issue 1