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Title: Ordered transport and identification of particles

Abstract

A method and apparatus are provided for application of electrical field gradients to induce particle velocities to enable particle sequence and identification information to be obtained. Particle sequence is maintained by providing electroosmotic flow for an electrolytic solution in a particle transport tube. The transport tube and electrolytic solution are selected to provide an electroosmotic radius of >100 so that a plug flow profile is obtained for the electrolytic solution in the transport tube. Thus, particles are maintained in the same order in which they are introduced in the transport tube. When the particles also have known electrophoretic velocities, the field gradients introduce an electrophoretic velocity component onto the electroosmotic velocity. The time that the particles pass selected locations along the transport tube may then be detected and the electrophoretic velocity component calculated for particle identification. One particular application is the ordered transport and identification of labeled nucleotides sequentially cleaved from a strand of DNA.

Inventors:
 [1]
  1. (Los Alamos, NM)
Issue Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM
OSTI Identifier:
868780
Patent Number(s):
5209834
Assignee:
United States of America as represented by United States (Washington, DC) LANL
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
transport; identification; particles; method; apparatus; provided; application; electrical; field; gradients; induce; particle; velocities; enable; sequence; information; obtained; maintained; providing; electroosmotic; flow; electrolytic; solution; tube; selected; provide; radius; 100; plug; profile; introduced; electrophoretic; introduce; velocity; component; time; pass; locations; detected; calculated; particular; labeled; nucleotides; sequentially; cleaved; strand; dna; velocity component; transport tube; field gradient; field gradients; particle transport; electrical field; electrolytic solution; particular application; selected location; selected locations; particles pass; identification information; particle velocities; particle sequence; particle identification; labeled nucleotides; /204/

Citation Formats

Shera, E. Brooks. Ordered transport and identification of particles. United States: N. p., 1993. Web.
Shera, E. Brooks. Ordered transport and identification of particles. United States.
Shera, E. Brooks. Fri . "Ordered transport and identification of particles". United States. https://www.osti.gov/servlets/purl/868780.
@article{osti_868780,
title = {Ordered transport and identification of particles},
author = {Shera, E. Brooks},
abstractNote = {A method and apparatus are provided for application of electrical field gradients to induce particle velocities to enable particle sequence and identification information to be obtained. Particle sequence is maintained by providing electroosmotic flow for an electrolytic solution in a particle transport tube. The transport tube and electrolytic solution are selected to provide an electroosmotic radius of >100 so that a plug flow profile is obtained for the electrolytic solution in the transport tube. Thus, particles are maintained in the same order in which they are introduced in the transport tube. When the particles also have known electrophoretic velocities, the field gradients introduce an electrophoretic velocity component onto the electroosmotic velocity. The time that the particles pass selected locations along the transport tube may then be detected and the electrophoretic velocity component calculated for particle identification. One particular application is the ordered transport and identification of labeled nucleotides sequentially cleaved from a strand of DNA.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {1}
}

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