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Title: Regioselective chemical modification of monoclonal antibodies

Abstract

A method of selectively modifying an immunoglobulin having at least one Fab region and at least one Fc region, each region having an isoelectric point wherein said isoelectric point of the Fab fragment of said immunoglobulin is different than the isoelectric point of the Fc fragment of the immunoglobulin, said method comprising modification of the immunoglobulin at a pH between the respective isoelectric points of the Fab and Fc fragments of the immunoglobulin.

Inventors:
 [1];  [1];  [1];  [1]
  1. Pittsburgh, PA
Issue Date:
Research Org.:
Univ. of Pittsburgh, PA (United States)
OSTI Identifier:
868770
Patent Number(s):
5208008
Assignee:
University of Pittsburgh (Pittsburgh, PA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
FG02-89ER60869
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
regioselective; chemical; modification; monoclonal; antibodies; method; selectively; modifying; immunoglobulin; fab; region; fc; isoelectric; fragment; comprising; ph; respective; fragments; method comprising; monoclonal antibodies; selective chemical; selectively modifying; chemical modification; /424/435/436/530/

Citation Formats

Ranadive, Girish, Rosenzweig, Howard S, Epperly, Michael, and Bloomer, William. Regioselective chemical modification of monoclonal antibodies. United States: N. p., 1993. Web.
Ranadive, Girish, Rosenzweig, Howard S, Epperly, Michael, & Bloomer, William. Regioselective chemical modification of monoclonal antibodies. United States.
Ranadive, Girish, Rosenzweig, Howard S, Epperly, Michael, and Bloomer, William. Fri . "Regioselective chemical modification of monoclonal antibodies". United States. https://www.osti.gov/servlets/purl/868770.
@article{osti_868770,
title = {Regioselective chemical modification of monoclonal antibodies},
author = {Ranadive, Girish and Rosenzweig, Howard S and Epperly, Michael and Bloomer, William},
abstractNote = {A method of selectively modifying an immunoglobulin having at least one Fab region and at least one Fc region, each region having an isoelectric point wherein said isoelectric point of the Fab fragment of said immunoglobulin is different than the isoelectric point of the Fc fragment of the immunoglobulin, said method comprising modification of the immunoglobulin at a pH between the respective isoelectric points of the Fab and Fc fragments of the immunoglobulin.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {1}
}

Works referenced in this record:

Inhibition of mannosidase in hybridomas yields monoclonal antibodies with greater capacity for carbohydrate labeling.
journal, September 1988


Linkage of cytotoxic agents to immunoglobulins
journal, January 1983


Strategies for designing antibody-toxin conjugates
journal, October 1988


Monoclonal antibodies with cytotoxic reactivities against human gliomas
journal, December 1989