Regioselective chemical modification of monoclonal antibodies
Abstract
A method of selectively modifying an immunoglobulin having at least one Fab region and at least one Fc region, each region having an isoelectric point wherein said isoelectric point of the Fab fragment of said immunoglobulin is different than the isoelectric point of the Fc fragment of the immunoglobulin, said method comprising modification of the immunoglobulin at a pH between the respective isoelectric points of the Fab and Fc fragments of the immunoglobulin.
- Inventors:
-
- Pittsburgh, PA
- Issue Date:
- Research Org.:
- Univ. of Pittsburgh, PA (United States)
- OSTI Identifier:
- 868770
- Patent Number(s):
- 5208008
- Assignee:
- University of Pittsburgh (Pittsburgh, PA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- FG02-89ER60869
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- regioselective; chemical; modification; monoclonal; antibodies; method; selectively; modifying; immunoglobulin; fab; region; fc; isoelectric; fragment; comprising; ph; respective; fragments; method comprising; monoclonal antibodies; selective chemical; selectively modifying; chemical modification; /424/435/436/530/
Citation Formats
Ranadive, Girish, Rosenzweig, Howard S, Epperly, Michael, and Bloomer, William. Regioselective chemical modification of monoclonal antibodies. United States: N. p., 1993.
Web.
Ranadive, Girish, Rosenzweig, Howard S, Epperly, Michael, & Bloomer, William. Regioselective chemical modification of monoclonal antibodies. United States.
Ranadive, Girish, Rosenzweig, Howard S, Epperly, Michael, and Bloomer, William. Fri .
"Regioselective chemical modification of monoclonal antibodies". United States. https://www.osti.gov/servlets/purl/868770.
@article{osti_868770,
title = {Regioselective chemical modification of monoclonal antibodies},
author = {Ranadive, Girish and Rosenzweig, Howard S and Epperly, Michael and Bloomer, William},
abstractNote = {A method of selectively modifying an immunoglobulin having at least one Fab region and at least one Fc region, each region having an isoelectric point wherein said isoelectric point of the Fab fragment of said immunoglobulin is different than the isoelectric point of the Fc fragment of the immunoglobulin, said method comprising modification of the immunoglobulin at a pH between the respective isoelectric points of the Fab and Fc fragments of the immunoglobulin.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1993},
month = {Fri Jan 01 00:00:00 EST 1993}
}
Works referenced in this record:
Inhibition of mannosidase in hybridomas yields monoclonal antibodies with greater capacity for carbohydrate labeling.
journal, September 1988
- Simonson, R. B.; Ultee, M. E.; Long, C. G.
- Clinical Chemistry, Vol. 34, Issue 9
Linkage of cytotoxic agents to immunoglobulins
journal, January 1983
- Huntley Blair, A.; Ghose, Tarun I.
- Journal of Immunological Methods, Vol. 59, Issue 2
Strategies for designing antibody-toxin conjugates
journal, October 1988
- Ahmad, Ateeq; Law, Kevin
- Trends in Biotechnology, Vol. 6, Issue 10
Monoclonal antibodies with cytotoxic reactivities against human gliomas
journal, December 1989
- Nanda, Anil; Liwnicz, Boleslaw; Atkinson, Barbara F.
- Journal of Neurosurgery, Vol. 71, Issue 6
[20] Preparation of antibody-linked cytotoxic agents
book, January 1983
- Ghose, Tarun I.; Huntley Blair, A.; Kulkarni, Padmaja N.
- Immunochemical Techniques Part F: Conventional Antibodies, Fc Receptors, and Cytotoxicity