Means and method of detection in chemical separation procedures
Abstract
A means and method for indirect detection of constituent components of a mixture separated in a chemical separation process. Fluorescing ions are distributed across the area in which separation of the mixture will occur to provide a generally uniform background fluorescence intensity. For example, the mixture is comprised of one or more charged analytes which displace fluorescing ions where its constituent components separate to. Fluorescing ions of the same charge as the charged analyte components cause a displacement. The displacement results in the location of the separated components having a reduced fluorescence intensity to the remainder of the background. Detection of the lower fluorescence intensity areas can be visually, by photographic means and methods, or by automated laser scanning.
- Inventors:
-
- Ames, IA
- Issue Date:
- Research Org.:
- Ames Laboratory (AMES), Ames, IA; Iowa State Univ., Ames, IA (United States)
- OSTI Identifier:
- 868691
- Patent Number(s):
- 5192407
- Application Number:
- 07/472,315
- Assignee:
- Iowa State University Research Foundation, Inc. (Ames, IA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-82
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- means; method; detection; chemical; separation; procedures; indirect; constituent; components; mixture; separated; process; fluorescing; distributed; occur; provide; uniform; background; fluorescence; intensity; example; comprised; charged; analytes; displace; separate; charge; analyte; displacement; results; location; reduced; remainder; visually; photographic; methods; automated; laser; scanning; direct detection; separation process; fluorescence intensity; separated components; chemical separation; laser scanning; constituent components; mixture separated; laser scan; /204/
Citation Formats
Yeung, Edward S, Koutny, Lance B, Hogan, Barry L, Cheung, Chan K, and Ma, Yinfa. Means and method of detection in chemical separation procedures. United States: N. p., 1993.
Web.
Yeung, Edward S, Koutny, Lance B, Hogan, Barry L, Cheung, Chan K, & Ma, Yinfa. Means and method of detection in chemical separation procedures. United States.
Yeung, Edward S, Koutny, Lance B, Hogan, Barry L, Cheung, Chan K, and Ma, Yinfa. Fri .
"Means and method of detection in chemical separation procedures". United States. https://www.osti.gov/servlets/purl/868691.
@article{osti_868691,
title = {Means and method of detection in chemical separation procedures},
author = {Yeung, Edward S and Koutny, Lance B and Hogan, Barry L and Cheung, Chan K and Ma, Yinfa},
abstractNote = {A means and method for indirect detection of constituent components of a mixture separated in a chemical separation process. Fluorescing ions are distributed across the area in which separation of the mixture will occur to provide a generally uniform background fluorescence intensity. For example, the mixture is comprised of one or more charged analytes which displace fluorescing ions where its constituent components separate to. Fluorescing ions of the same charge as the charged analyte components cause a displacement. The displacement results in the location of the separated components having a reduced fluorescence intensity to the remainder of the background. Detection of the lower fluorescence intensity areas can be visually, by photographic means and methods, or by automated laser scanning.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {1}
}
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Works referenced in this record:
Indirect Fluorometric Detection in Gel Electrophoresis
journal, February 1989
- Hogan, Barry L.; Yeung, Edward S.
- Applied Spectroscopy, Vol. 43, Issue 2
Indirect fluorimetric detection of non-electrolytes in thin-layer chromatography
journal, January 1988
- Ma, Yinfa; Yeung, Edward S.
- Journal of Chromatography A, Vol. 455
Laser-based indirect fluorometric detection and quantification in thin-layer chromatography
journal, September 1989
- Ma, Yinfa.; Koutny, Lance B.; Yeung, Edward S.
- Analytical Chemistry, Vol. 61, Issue 17
Indirect fluorometric detection of anions in thin-layer chromatography
journal, April 1988
- Ma, Yinfa.; Yeung, Edward S.
- Analytical Chemistry, Vol. 60, Issue 7