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Title: cDNA encoding a polypeptide including a hevein sequence

Abstract

A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a pu GOVERNMENT RIGHTS This application was funded under Department of Energy Contract DE-AC02-76ER01338. The U.S. Government has certain rights under this application and any patent issuing thereon.

Inventors:
 [1];  [2];  [3];  [4]
  1. Okemos, MI
  2. Dilbeek, BE
  3. Scarsdale, NY
  4. New York, NY
Issue Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States)
OSTI Identifier:
868663
Patent Number(s):
5187262
Application Number:
07/587,071
Assignee:
Board of Trustees, operating Michigan State University (East Lansing, MI)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
AC02-76ER01338
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
cdna; encoding; polypeptide; including; hevein; sequence; clone; hev1; isolated; via; polymerase; chain; reaction; pcr; mixed; oligonucleotides; corresponding; regions; primers; hevea; brasiliensis; latex; library; template; 1018; nucleotides; reading; frame; 204; amino; acids; deduced; acid; contains; government; rights; application; funded; department; energy; contract; de-ac02-76er01338; issuing; thereon; cdna clone; deduced amino; reading frame; chain reaction; acid sequence; amino acid; polymerase chain; amino acids; cdna library; dna encoding; energy contract; cdna encoding; sequence contains; oligonucleotides corresponding; mixed oligonucleotides; via polymerase; encoding hevein; hevea brasiliensis; government rights; brasiliensis latex; latex cdna; isolated via; polypeptide including; hevein sequence; nucleotides corresponding; /530/435/

Citation Formats

Raikhel, Natasha V, Broekaert, Willem F, Chua, Nam-Hai, and Kush, Anil. cDNA encoding a polypeptide including a hevein sequence. United States: N. p., 1993. Web.
Raikhel, Natasha V, Broekaert, Willem F, Chua, Nam-Hai, & Kush, Anil. cDNA encoding a polypeptide including a hevein sequence. United States.
Raikhel, Natasha V, Broekaert, Willem F, Chua, Nam-Hai, and Kush, Anil. Tue . "cDNA encoding a polypeptide including a hevein sequence". United States. https://www.osti.gov/servlets/purl/868663.
@article{osti_868663,
title = {cDNA encoding a polypeptide including a hevein sequence},
author = {Raikhel, Natasha V and Broekaert, Willem F and Chua, Nam-Hai and Kush, Anil},
abstractNote = {A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a pu GOVERNMENT RIGHTS This application was funded under Department of Energy Contract DE-AC02-76ER01338. The U.S. Government has certain rights under this application and any patent issuing thereon.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {2}
}

Works referenced in this record:

Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP
journal, January 1986


Comparison of some molecular, enzymatic and antifungal properties of chitinases from thorn-apple, tobacco and wheat
journal, November 1988


Primary structure of wheat germ agglutinin isolectin 2. Peptide order deduced from x-ray structure
journal, January 1984


Host-Pathogen Interactions: XVIII. ISOLATION AND BIOLOGICAL ACTIVITY OF GLYCINOL, A PTEROCARPAN PHYTOALEXIN SYNTHESIZED BY SOYBEANS
journal, August 1981


Ethylene Biosynthesis and its Regulation in Higher Plants
journal, June 1984


Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris.
journal, September 1986


Cloning and Characterization of Root-Specific Barley Lectin
journal, September 1989


DNA sequencing with chain-terminating inhibitors
journal, December 1977


Représentations sociales des causes de la délinquance : Croyances générales et cas concrets
journal, January 1987


Regulation of wound ethylene synthesis in plants
journal, July 1980


A simple and very efficient method for generating cDNA libraries
journal, November 1983


Sequence complexity of polyadenylated ribonucleic acid from soybean suspension culture cells
journal, June 1979


A rapid and sensitive assay for chitinase using tritiated chitin
journal, December 1977


Plant chitinases are potent inhibitors of fungal growth
journal, November 1986


Regulation of synthesis of proteinase inhibitors I and II mRNAs in leaves of wounded tomato plants
journal, November 1986


Chitinase cDNA Cloning and mRNA Induction by Fungal Elicitor, Wounding, and Infection
journal, January 1988


Cell-type-specific expression of a wheat-germ agglutinin gene in embryos and young seedlings of Triticum aestivum
journal, December 1988


A Chitin-Binding Lectin from Stinging Nettle Rhizomes with Antifungal Properties
journal, September 1989


Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato
journal, December 1989


Extensive homologies between lectins from non-leguminous plants
journal, January 1986


Homology between chitinases that are induced by TMV infection of tobacco
journal, January 1987


Effect of Ethylene Action Inhibitors upon Wound-Induced Gene Expression in Tomato Pericarp
journal, September 1989


Plant defense genes are regulated by ethylene
journal, August 1987


Differential expression within a family of novel wound-induced genes in potato
journal, January 1989