Method for distinguishing normal and transformed cells using G1 kinase inhibitors
Abstract
A G.sub.1 phase kinase inhibitor is applied in a low concentration to a population of normal and transformed mammalian cells. The concentration of G.sub.1 phase kinase inhibitor is selected to reversibly arrest normal mammalian cells in the G.sub.1 cell cycle without arresting growth of transformed cells. The transformed cells may then be selectively identified and/or cloned for research or diagnostic purposes. The transformed cells may also be selectively killed by therapeutic agents that do not affect normal cells in the G.sub.1 phase, suggesting that such G.sub.1 phase kinase inhibitors may form an effective adjuvant for use with chemotherapeutic agents in cancer therapy for optimizing the killing dose of chemotherapeutic agents while minimizing undesirable side effects on normal cells.
- Inventors:
-
- (Los Alamos, NM)
- (Santa Fe, NM)
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM
- OSTI Identifier:
- 868655
- Patent Number(s):
- 5185260
- Assignee:
- United States of America as represented by United States (Washington, DC) LANL
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; distinguishing; normal; transformed; cells; g1; kinase; inhibitors; phase; inhibitor; applied; concentration; population; mammalian; selected; reversibly; arrest; cell; cycle; arresting; growth; selectively; identified; cloned; diagnostic; purposes; killed; therapeutic; agents; affect; suggesting; form; effective; adjuvant; chemotherapeutic; cancer; therapy; optimizing; killing; dose; minimizing; undesirable; effects; therapeutic agents; normal cells; cancer therapy; cell cycle; mammalian cells; therapeutic agent; phase kinase; diagnostic purposes; kinase inhibitor; /435/424/999/
Citation Formats
Crissman, Harry A., Gadbois, Donna M., Tobey, Robert A., and Bradbury, E. Morton. Method for distinguishing normal and transformed cells using G1 kinase inhibitors. United States: N. p., 1993.
Web.
Crissman, Harry A., Gadbois, Donna M., Tobey, Robert A., & Bradbury, E. Morton. Method for distinguishing normal and transformed cells using G1 kinase inhibitors. United States.
Crissman, Harry A., Gadbois, Donna M., Tobey, Robert A., and Bradbury, E. Morton. Fri .
"Method for distinguishing normal and transformed cells using G1 kinase inhibitors". United States. https://www.osti.gov/servlets/purl/868655.
@article{osti_868655,
title = {Method for distinguishing normal and transformed cells using G1 kinase inhibitors},
author = {Crissman, Harry A. and Gadbois, Donna M. and Tobey, Robert A. and Bradbury, E. Morton},
abstractNote = {A G.sub.1 phase kinase inhibitor is applied in a low concentration to a population of normal and transformed mammalian cells. The concentration of G.sub.1 phase kinase inhibitor is selected to reversibly arrest normal mammalian cells in the G.sub.1 cell cycle without arresting growth of transformed cells. The transformed cells may then be selectively identified and/or cloned for research or diagnostic purposes. The transformed cells may also be selectively killed by therapeutic agents that do not affect normal cells in the G.sub.1 phase, suggesting that such G.sub.1 phase kinase inhibitors may form an effective adjuvant for use with chemotherapeutic agents in cancer therapy for optimizing the killing dose of chemotherapeutic agents while minimizing undesirable side effects on normal cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {1}
}