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Title: Multiplex analysis of DNA

Abstract

This invention features vectors and a method for sequencing DNA. The method includes the steps of: a) ligating the DNA into a vector comprising a tag sequence, the tag sequence includes at least 15 bases, wherein the tag sequence will not hybridize to the DNA under stringent hybridization conditions and is unique in the vector, to form a hybrid vector, b) treating the hybrid vector in a plurality of vessels to produce fragments comprising the tag sequence, wherein the fragments differ in length and terminate at a fixed known base or bases, wherein the fixed known base or bases differs in each vessel, c) separating the fragments from each vessel according to their size, d) hybridizing the fragments with an oligonucleotide able to hybridize specifically with the tag sequence, and e) detecting the pattern of hybridization of the tag sequence, wherein the pattern reflects the nucleotide sequence of the DNA.

Inventors:
 [1];  [2]
  1. (Boston, MA)
  2. (Dorchester, MA)
Issue Date:
Research Org.:
Harvard University
OSTI Identifier:
868474
Patent Number(s):
5149625
Assignee:
President and Fellows of Harvard College (Cambridge, MA) CHO
DOE Contract Number:  
FG02-87ER60565
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
multiplex; analysis; dna; features; vectors; method; sequencing; steps; ligating; vector; comprising; tag; sequence; 15; bases; hybridize; stringent; hybridization; conditions; unique; form; hybrid; treating; plurality; vessels; produce; fragments; length; terminate; fixed; base; differs; vessel; separating; according; size; hybridizing; oligonucleotide; specifically; detecting; pattern; reflects; nucleotide; tag sequence; sequencing dna; nucleotide sequence; vector comprising; /435/436/

Citation Formats

Church, George M., and Kieffer-Higgins, Stephen. Multiplex analysis of DNA. United States: N. p., 1992. Web.
Church, George M., & Kieffer-Higgins, Stephen. Multiplex analysis of DNA. United States.
Church, George M., and Kieffer-Higgins, Stephen. Wed . "Multiplex analysis of DNA". United States. https://www.osti.gov/servlets/purl/868474.
@article{osti_868474,
title = {Multiplex analysis of DNA},
author = {Church, George M. and Kieffer-Higgins, Stephen},
abstractNote = {This invention features vectors and a method for sequencing DNA. The method includes the steps of: a) ligating the DNA into a vector comprising a tag sequence, the tag sequence includes at least 15 bases, wherein the tag sequence will not hybridize to the DNA under stringent hybridization conditions and is unique in the vector, to form a hybrid vector, b) treating the hybrid vector in a plurality of vessels to produce fragments comprising the tag sequence, wherein the fragments differ in length and terminate at a fixed known base or bases, wherein the fixed known base or bases differs in each vessel, c) separating the fragments from each vessel according to their size, d) hybridizing the fragments with an oligonucleotide able to hybridize specifically with the tag sequence, and e) detecting the pattern of hybridization of the tag sequence, wherein the pattern reflects the nucleotide sequence of the DNA.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1992},
month = {1}
}

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