Title: DNA Sequencing apparatus

Abstract

An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

Inventors:

Tabor, Stanley ^[1]; Richardson, Charles C ^[2]

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1. Cambridge, MA
2. Chestnut Hill, MA

Issue Date:

1992-01-01

Research Org.:

Harvard University

OSTI Identifier:

868349

Patent Number(s):

5122345

Assignee:

President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q2527/137 - Concentration of a component of medium

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S435/81 - Packaged device or kit

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

Y10T436/143333 - Saccharide [e.g., DNA, etc.]

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6869 - Methods for sequencing
C12Q2535/101 - Sanger sequencing method, i.e. oligonucleotide sequencing using primer elongation and dideoxynucleotides as chain terminators
C12Q2537/157 - A reaction step characterised by the number of molecules incorporated or released

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S435/803 - Physical recovery methods, e.g. chromatography, grinding
Y10S436/808 - Automated or kit

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q2521/125 - Methyl transferase, i.e. methylase

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DOE Contract Number:  

FG02-88ER60688

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

dna; sequencing; apparatus; automated; reactor; providing; series; products; formed; single; primer; strand; product; differing; molecular; weight; chain; terminating; agent; separating; means; form; bands; intensity; substantially; nearby; band; reading; determining; position; government; support; including; grant; public; health; service; contract; ai-06045; rights; dna strand; molecular weight; dna sequencing; government support; support including; sequencing apparatus; products formed; /422/204/