DNA Sequencing apparatus

Title: DNA Sequencing apparatus

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Abstract

An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

Inventors:

Tabor, Stanley ^[1]; Richardson, Charles C ^[2]

Cambridge, MA
Chestnut Hill, MA

Issue Date:: 1992-01-01

Research Org.:: Harvard University

OSTI Identifier:: 868349

Patent Number(s):: 5122345

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q2527/137 - Concentration of a component of medium

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S435/81 - Packaged device or kit

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
Y10T436/143333 - Saccharide [e.g., DNA, etc.]

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/6869 - Methods for sequencing
C12Q2535/101 - Sanger sequencing method, i.e. oligonucleotide sequencing using primer elongation and dideoxynucleotides as chain terminators
C12Q2537/157 - A reaction step characterised by the number of molecules incorporated or released

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S435/803 - Physical recovery methods, e.g. chromatography, grinding
Y10S436/808 - Automated or kit

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q2521/125 - Methyl transferase, i.e. methylase

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DOE Contract Number:: FG02-88ER60688

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: dna; sequencing; apparatus; automated; reactor; providing; series; products; formed; single; primer; strand; product; differing; molecular; weight; chain; terminating; agent; separating; means; form; bands; intensity; substantially; nearby; band; reading; determining; position; government; support; including; grant; public; health; service; contract; ai-06045; rights; dna strand; molecular weight; dna sequencing; government support; support including; sequencing apparatus; products formed; /422/204/

Citation Formats


                    Tabor, Stanley, and Richardson, Charles C. DNA Sequencing apparatus.  United States: N. p., 1992. 
        Web.

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                    Tabor, Stanley, & Richardson, Charles C. DNA Sequencing apparatus.  United States.

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                    Tabor, Stanley, and Richardson, Charles C. Wed .  
        "DNA Sequencing apparatus".  United States.  https://www.osti.gov/servlets/purl/868349.

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@article{osti_868349,

  title        = {DNA Sequencing apparatus},

  author       = {Tabor, Stanley and Richardson, Charles C},

  abstractNote = {An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1992},

  month        = {1}

}

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