Integrated fluorescence analysis system
Abstract
An integrated fluorescence analysis system enables a component part of a sample to be virtually sorted within a sample volume after a spectrum of the component part has been identified from a fluorescence spectrum of the entire sample in a flow cytometer. Birefringent optics enables the entire spectrum to be resolved into a set of numbers representing the intensity of spectral components of the spectrum. One or more spectral components are selected to program a scanning laser microscope, preferably a confocal microscope, whereby the spectrum from individual pixels or voxels in the sample can be compared. Individual pixels or voxels containing the selected spectral components are identified and an image may be formed to show the morphology of the sample with respect to only those components having the selected spectral components. There is no need for any physical sorting of the sample components to obtain the morphological information.
- Inventors:
-
- Los Alamos, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- OSTI Identifier:
- 868318
- Patent Number(s):
- 5117466
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- integrated; fluorescence; analysis; enables; component; sample; virtually; sorted; volume; spectrum; identified; entire; flow; cytometer; birefringent; optics; resolved; set; representing; intensity; spectral; components; selected; scanning; laser; microscope; preferably; confocal; whereby; individual; pixels; voxels; compared; containing; image; formed; morphology; respect; physical; sorting; obtain; morphological; information; selected spectral; confocal microscope; flow cytometer; spectral components; scanning laser; sample volume; individual pixels; fluorescence spectrum; laser microscope; sample components; fluorescence analysis; integrated fluorescence; spectral component; /382/250/356/
Citation Formats
Buican, Tudor N, and Yoshida, Thomas M. Integrated fluorescence analysis system. United States: N. p., 1992.
Web.
Buican, Tudor N, & Yoshida, Thomas M. Integrated fluorescence analysis system. United States.
Buican, Tudor N, and Yoshida, Thomas M. Wed .
"Integrated fluorescence analysis system". United States. https://www.osti.gov/servlets/purl/868318.
@article{osti_868318,
title = {Integrated fluorescence analysis system},
author = {Buican, Tudor N and Yoshida, Thomas M},
abstractNote = {An integrated fluorescence analysis system enables a component part of a sample to be virtually sorted within a sample volume after a spectrum of the component part has been identified from a fluorescence spectrum of the entire sample in a flow cytometer. Birefringent optics enables the entire spectrum to be resolved into a set of numbers representing the intensity of spectral components of the spectrum. One or more spectral components are selected to program a scanning laser microscope, preferably a confocal microscope, whereby the spectrum from individual pixels or voxels in the sample can be compared. Individual pixels or voxels containing the selected spectral components are identified and an image may be formed to show the morphology of the sample with respect to only those components having the selected spectral components. There is no need for any physical sorting of the sample components to obtain the morphological information.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1992},
month = {1}
}