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Title: Thermostable purified endoglucanase from thermophilic bacterium acidothermus cellulolyticus

A substantially purified high molecular weight cellulase enzyme having a molecular weight of between about 156,000 to about 203,400 daltons isolated from the bacterium Acidothermus cellulolyticus (ATCC 43068) and a method of producing it are disclosed. The enzyme is water soluble, possesses both C.sub.1 and C.sub.x types of enzymatic activity, has a high degree of stability toward heat and exhibits both a high optimum temperature activity and high inactivation characteristics.
Inventors:
 [1];  [2];  [2];  [3]
  1. (Lakewood, CO)
  2. (Littleton, CO)
  3. (Golden, CO)
Issue Date:
OSTI Identifier:
868277
Assignee:
Midwest Research Institute (Kansas City, MO) NREL
Patent Number(s):
US 5110735
Contract Number:
AC02-83CH10093
Research Org:
MIDWEST RESEARCH INSTITUTE
Country of Publication:
United States
Language:
English
Subject:
thermostable; purified; endoglucanase; thermophilic; bacterium; acidothermus; cellulolyticus; substantially; molecular; weight; cellulase; enzyme; 156; 000; 203; 400; daltons; isolated; atcc; 43068; method; producing; disclosed; water; soluble; possesses; types; enzymatic; activity; degree; stability; heat; exhibits; optimum; temperature; inactivation; characteristics; cellulase enzyme; optimum temperature; water soluble; molecular weight; acidothermus cellulolyticus; thermostable purified; temperature activity; purified endoglucanase; enzymatic activity; weight cellulase; purified endoglucanas; /435/