Thermostable purified endoglucanase from thermophilic bacterium acidothermus cellulolyticus

Title: Thermostable purified endoglucanase from thermophilic bacterium acidothermus cellulolyticus

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A substantially purified high molecular weight cellulase enzyme having a molecular weight of between about 156,000 to about 203,400 daltons isolated from the bacterium Acidothermus cellulolyticus (ATCC 43068) and a method of producing it are disclosed. The enzyme is water soluble, possesses both C.sub.1 and C.sub.x types of enzymatic activity, has a high degree of stability toward heat and exhibits both a high optimum temperature activity and high inactivation characteristics.

Inventors:

Tucker, Melvin P. ^[1]; Grohmann, Karel ^[2]; Himmel, Michael E. ^[2]; Mohagheghi, Ali ^[3]

(Lakewood, CO)
(Littleton, CO)
(Golden, CO)

Issue Date:: 1992-01-01

OSTI Identifier:: 868277

Assignee:: Midwest Research Institute (Kansas City, MO) NREL

Patent Number(s):: US 5110735

Contract Number:: AC02-83CH10093

Research Org:: MIDWEST RESEARCH INSTITUTE

Country of Publication:: United States

Language:: English

Subject:: thermostable; purified; endoglucanase; thermophilic; bacterium; acidothermus; cellulolyticus; substantially; molecular; weight; cellulase; enzyme; 156; 000; 203; 400; daltons; isolated; atcc; 43068; method; producing; disclosed; water; soluble; possesses; types; enzymatic; activity; degree; stability; heat; exhibits; optimum; temperature; inactivation; characteristics; cellulase enzyme; optimum temperature; water soluble; molecular weight; acidothermus cellulolyticus; thermostable purified; temperature activity; purified endoglucanase; enzymatic activity; weight cellulase; purified endoglucanas; /435/

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