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Title: Thermostable purified endoglucanase from thermophilic bacterium acidothermus cellulolyticus

Abstract

A substantially purified high molecular weight cellulase enzyme having a molecular weight of between about 156,000 to about 203,400 daltons isolated from the bacterium Acidothermus cellulolyticus (ATCC 43068) and a method of producing it are disclosed. The enzyme is water soluble, possesses both C.sub.1 and C.sub.x types of enzymatic activity, has a high degree of stability toward heat and exhibits both a high optimum temperature activity and high inactivation characteristics.

Inventors:
 [1];  [2];  [2];  [3]
  1. Lakewood, CO
  2. Littleton, CO
  3. Golden, CO
Issue Date:
Research Org.:
Midwest Research Institute, Kansas City, MO (United States)
OSTI Identifier:
868277
Patent Number(s):
5110735
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC02-83CH10093
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
thermostable; purified; endoglucanase; thermophilic; bacterium; acidothermus; cellulolyticus; substantially; molecular; weight; cellulase; enzyme; 156; 000; 203; 400; daltons; isolated; atcc; 43068; method; producing; disclosed; water; soluble; possesses; types; enzymatic; activity; degree; stability; heat; exhibits; optimum; temperature; inactivation; characteristics; cellulase enzyme; optimum temperature; water soluble; molecular weight; acidothermus cellulolyticus; thermostable purified; temperature activity; purified endoglucanase; enzymatic activity; weight cellulase; purified endoglucanas; /435/

Citation Formats

Tucker, Melvin P, Grohmann, Karel, Himmel, Michael E, and Mohagheghi, Ali. Thermostable purified endoglucanase from thermophilic bacterium acidothermus cellulolyticus. United States: N. p., 1992. Web.
Tucker, Melvin P, Grohmann, Karel, Himmel, Michael E, & Mohagheghi, Ali. Thermostable purified endoglucanase from thermophilic bacterium acidothermus cellulolyticus. United States.
Tucker, Melvin P, Grohmann, Karel, Himmel, Michael E, and Mohagheghi, Ali. Wed . "Thermostable purified endoglucanase from thermophilic bacterium acidothermus cellulolyticus". United States. https://www.osti.gov/servlets/purl/868277.
@article{osti_868277,
title = {Thermostable purified endoglucanase from thermophilic bacterium acidothermus cellulolyticus},
author = {Tucker, Melvin P and Grohmann, Karel and Himmel, Michael E and Mohagheghi, Ali},
abstractNote = {A substantially purified high molecular weight cellulase enzyme having a molecular weight of between about 156,000 to about 203,400 daltons isolated from the bacterium Acidothermus cellulolyticus (ATCC 43068) and a method of producing it are disclosed. The enzyme is water soluble, possesses both C.sub.1 and C.sub.x types of enzymatic activity, has a high degree of stability toward heat and exhibits both a high optimum temperature activity and high inactivation characteristics.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1992},
month = {1}
}

Works referenced in this record:

Cellulolytic enzyme system of Acetivibrio cellulolyticus
journal, March 1981


The Production of Cellulases
book, June 1969


Mechanisms of Thermophily
journal, January 1978


Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria
journal, July 1986