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Title: Optical probe for the cytochrome P-450 cholesterol side chain cleavage enzyme

Abstract

An optical probe enables the study of enzyme activity by absorbance spectroscopy or by sensitive fluorescence methods. In particular, the probe provides the ability to monitor the activity of cytochrome P-450.sub.scc enzyme, the rate limiting enzyme for steroid biosynthesis. Located on the inner mitochondrial membrane, P-450.sub.scc catalyzes the conversion of cholesterol to pregnenolone and isocapraldehyde by sequential oxidations of the cholesterol side chain. The fluorogenic probe includes a cholesterol-like steroid linked to a chromophore through a linking group. The chromophore is selected to have little optical response when linked to the steroid substrate and an enhanced optical response when cleaved from the substrate and linking group. Thus, a fluorescent anion that can be optically detected is generated by the side-chain cleavage reaction during steroidogenesis.

Inventors:
 [1];  [1];  [1];  [2]
+ Show Inventor Affiliations
  1. Los Alamos, NM
  2. Santa Fe, NM
Issue Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
OSTI Identifier:
868276
Patent Number(s):
5110725
Assignee:
United States of America as represented by United States (Washington, DC)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
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DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
optical; probe; cytochrome; p-450; cholesterol; chain; cleavage; enzyme; enables; study; activity; absorbance; spectroscopy; sensitive; fluorescence; methods; particular; provides; ability; monitor; scc; rate; limiting; steroid; biosynthesis; located; inner; mitochondrial; membrane; catalyzes; conversion; pregnenolone; isocapraldehyde; sequential; oxidations; fluorogenic; cholesterol-like; linked; chromophore; linking; selected; response; substrate; enhanced; cleaved; fluorescent; anion; optically; detected; generated; side-chain; reaction; steroidogenesis; enzyme activity; optical response; optical probe; optically detected; cytochrome p-450; probe provides; sensitive fluorescence; chain cleavage; cleavage enzyme; p-450 cholesterol; fluorescence method; fluorescence methods; probe enables; absorbance spectroscopy; /435/

Citation Formats

Marrone, Babetta L, Simpson, Daniel J, Unkefer, Clifford J, and Whaley, Thomas W. Optical probe for the cytochrome P-450 cholesterol side chain cleavage enzyme. United States: N. p., 1992. Web.
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Marrone, Babetta L, Simpson, Daniel J, Unkefer, Clifford J, & Whaley, Thomas W. Optical probe for the cytochrome P-450 cholesterol side chain cleavage enzyme. United States.
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Marrone, Babetta L, Simpson, Daniel J, Unkefer, Clifford J, and Whaley, Thomas W. Wed . "Optical probe for the cytochrome P-450 cholesterol side chain cleavage enzyme". United States. https://www.osti.gov/servlets/purl/868276.
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@article{osti_868276,
title = {Optical probe for the cytochrome P-450 cholesterol side chain cleavage enzyme},
author = {Marrone, Babetta L and Simpson, Daniel J and Unkefer, Clifford J and Whaley, Thomas W},
abstractNote = {An optical probe enables the study of enzyme activity by absorbance spectroscopy or by sensitive fluorescence methods. In particular, the probe provides the ability to monitor the activity of cytochrome P-450.sub.scc enzyme, the rate limiting enzyme for steroid biosynthesis. Located on the inner mitochondrial membrane, P-450.sub.scc catalyzes the conversion of cholesterol to pregnenolone and isocapraldehyde by sequential oxidations of the cholesterol side chain. The fluorogenic probe includes a cholesterol-like steroid linked to a chromophore through a linking group. The chromophore is selected to have little optical response when linked to the steroid substrate and an enhanced optical response when cleaved from the substrate and linking group. Thus, a fluorescent anion that can be optically detected is generated by the side-chain cleavage reaction during steroidogenesis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1992},
month = {1}
}
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Works referenced in this record:

Synthesis from pregnenolone of fluorescent cholesterol analog probes with conjugated unsaturation in the side chain
journal, September 1987

Simple and precise assay of the enzymic conversion of cholesterol into pregnenolone
journal, January 1974

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