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Title: Enzyme electrochemical sensor electrode and method of making it

Abstract

An electrochemical sensor electrode is formed from an electronic conductor coated with a casting solution containing a perfluorosulfonic acid ionomer and a selected enzyme. The selected enzyme catalyzes a reaction between a predetermined substance in a solution and oxygen to form an electrochemically active compound that is detected at the electronic conductor. The resulting perfluorosulfonic acid polymer provides a stable matrix for the enzyme for long lived enzyme activity, wherein only thin coatings are required on the metal conductor. The polymer also advantageously repels interfering substances from contacting the enzyme and contains quantities of oxygen to maintain a sensing capability during conditions of oxygen depletion in the sample. In one particular embodiment, glucose oxidase is mixed with the perfluorosulfonic acid ionomer to form an electrode for glucose detection.

Inventors:
 [1];  [1];  [1]
  1. Los Alamos, NM
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
OSTI Identifier:
868138
Patent Number(s):
5082550
Assignee:
United States of America as represented by Department of Energy (Washington, DC)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
enzyme; electrochemical; sensor; electrode; method; formed; electronic; conductor; coated; casting; solution; containing; perfluorosulfonic; acid; ionomer; selected; catalyzes; reaction; predetermined; substance; oxygen; form; electrochemically; active; compound; detected; resulting; polymer; provides; stable; matrix; lived; activity; coatings; required; metal; advantageously; repels; interfering; substances; contacting; contains; quantities; maintain; sensing; capability; conditions; depletion; sample; particular; embodiment; glucose; oxidase; mixed; detection; acid polymer; perfluorosulfonic acid; enzyme activity; sensor electrode; particular embodiment; electrochemical sensor; sulfonic acid; chemical sensor; metal conductor; solution containing; electronic conductor; casting solution; electrochemically active; acid ionomer; polymer provides; chemically active; active compound; selected enzyme; enzyme catalyzes; metal conduct; /204/435/

Citation Formats

Rishpon, Judith, Zawodzinski, Thomas A, and Gottesfeld, Shimshon. Enzyme electrochemical sensor electrode and method of making it. United States: N. p., 1992. Web.
Rishpon, Judith, Zawodzinski, Thomas A, & Gottesfeld, Shimshon. Enzyme electrochemical sensor electrode and method of making it. United States.
Rishpon, Judith, Zawodzinski, Thomas A, and Gottesfeld, Shimshon. Wed . "Enzyme electrochemical sensor electrode and method of making it". United States. https://www.osti.gov/servlets/purl/868138.
@article{osti_868138,
title = {Enzyme electrochemical sensor electrode and method of making it},
author = {Rishpon, Judith and Zawodzinski, Thomas A and Gottesfeld, Shimshon},
abstractNote = {An electrochemical sensor electrode is formed from an electronic conductor coated with a casting solution containing a perfluorosulfonic acid ionomer and a selected enzyme. The selected enzyme catalyzes a reaction between a predetermined substance in a solution and oxygen to form an electrochemically active compound that is detected at the electronic conductor. The resulting perfluorosulfonic acid polymer provides a stable matrix for the enzyme for long lived enzyme activity, wherein only thin coatings are required on the metal conductor. The polymer also advantageously repels interfering substances from contacting the enzyme and contains quantities of oxygen to maintain a sensing capability during conditions of oxygen depletion in the sample. In one particular embodiment, glucose oxidase is mixed with the perfluorosulfonic acid ionomer to form an electrode for glucose detection.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1992},
month = {1}
}

Patent:

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