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Title: Detection method for dissociation of multiple-charged ions

Abstract

Dissociations of multiple-charged ions are detected and analyzed by charge-separation tandem mass spectrometry. Analyte molecules are ionized to form multiple-charged parent ions. A particular charge parent ion state is selected in a first-stage mass spectrometer and its mass-to-charge ratio (M/Z) is detected to determine its mass and charge. The selected parent ions are then dissociated, each into a plurality of fragments including a set of daughter ions each having a mass of at least one molecular weight and a charge of at least one. Sets of daughter ions resulting from the dissociation of one parent ion (sibling ions) vary in number but typically include two to four ions, one or more multiply-charged. A second stage mass spectrometer detects mass-to-charge ratio (m/z) of the daughter ions and a temporal or temporo-spatial relationship among them. This relationship is used to correlate the daughter ions to determine which (m/z) ratios belong to a set of sibling ions. Values of mass and charge of each of the sibling ions are determined simultaneously from their respective (m/z) ratios such that the sibling ion charges are integers and sum to the parent ion charge.

Inventors:
 [1];  [1];  [1]
  1. Richland, WA
Issue Date:
Research Org.:
Battelle Memorial Institute, Columbus, OH (United States)
OSTI Identifier:
868095
Patent Number(s):
5073713
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
detection; method; dissociation; multiple-charged; dissociations; detected; analyzed; charge-separation; tandem; mass; spectrometry; analyte; molecules; ionized; form; parent; particular; charge; selected; first-stage; spectrometer; mass-to-charge; ratio; z; determine; dissociated; plurality; fragments; including; set; daughter; molecular; weight; sets; resulting; sibling; vary; typically; multiply-charged; stage; detects; temporal; temporo-spatial; relationship; correlate; ratios; belong; values; determined; simultaneously; respective; charges; integers; detection method; mass spectrometer; molecular weight; mass spectrometry; form multiple; /250/

Citation Formats

Smith, Richard D, Udseth, Harold R, and Rockwood, Alan L. Detection method for dissociation of multiple-charged ions. United States: N. p., 1991. Web.
Smith, Richard D, Udseth, Harold R, & Rockwood, Alan L. Detection method for dissociation of multiple-charged ions. United States.
Smith, Richard D, Udseth, Harold R, and Rockwood, Alan L. Tue . "Detection method for dissociation of multiple-charged ions". United States. https://www.osti.gov/servlets/purl/868095.
@article{osti_868095,
title = {Detection method for dissociation of multiple-charged ions},
author = {Smith, Richard D and Udseth, Harold R and Rockwood, Alan L},
abstractNote = {Dissociations of multiple-charged ions are detected and analyzed by charge-separation tandem mass spectrometry. Analyte molecules are ionized to form multiple-charged parent ions. A particular charge parent ion state is selected in a first-stage mass spectrometer and its mass-to-charge ratio (M/Z) is detected to determine its mass and charge. The selected parent ions are then dissociated, each into a plurality of fragments including a set of daughter ions each having a mass of at least one molecular weight and a charge of at least one. Sets of daughter ions resulting from the dissociation of one parent ion (sibling ions) vary in number but typically include two to four ions, one or more multiply-charged. A second stage mass spectrometer detects mass-to-charge ratio (m/z) of the daughter ions and a temporal or temporo-spatial relationship among them. This relationship is used to correlate the daughter ions to determine which (m/z) ratios belong to a set of sibling ions. Values of mass and charge of each of the sibling ions are determined simultaneously from their respective (m/z) ratios such that the sibling ion charges are integers and sum to the parent ion charge.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1991},
month = {1}
}