Chromosome specific repetitive DNA sequences
Abstract
A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).
- Inventors:
-
- Los Alamos, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- OSTI Identifier:
- 868065
- Patent Number(s):
- 5064948
- Assignee:
- United States of America as represented by Department of Energy (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- chromosome; specific; repetitive; dna; sequences; method; provided; determining; nucleotide; useful; forming; probe; identify; chromosomes; preferably; situ; hybridization; cell; embodiment; preferential; determined; library; recombinant; clones; families; identified; homology; sequence; respectively; belong; variant; selecting; pattern; genomic; dissimilar; shown; respective; selected; family; classified; nonspecific; sequenced; regions; result; contract; department; energy; w-7405-eng-36; dna sequences; dna sequence; nucleotide sequences; nucleotide sequence; recombinant dna; chromosome specific; situ hybridization; repetitive sequences; identify specific; specific chromosomes; specific chromosome; /999/435/436/
Citation Formats
Moyzis, Robert K, and Meyne, Julianne. Chromosome specific repetitive DNA sequences. United States: N. p., 1991.
Web.
Moyzis, Robert K, & Meyne, Julianne. Chromosome specific repetitive DNA sequences. United States.
Moyzis, Robert K, and Meyne, Julianne. Tue .
"Chromosome specific repetitive DNA sequences". United States. https://www.osti.gov/servlets/purl/868065.
@article{osti_868065,
title = {Chromosome specific repetitive DNA sequences},
author = {Moyzis, Robert K and Meyne, Julianne},
abstractNote = {A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1991},
month = {1}
}