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Title: Determination of a mutational spectrum

Abstract

A method of resolving (physically separating) mutant DNA from nonmutant DNA and a method of defining or establishing a mutational spectrum or profile of alterations present in nucleic acid sequences from a sample to be analyzed, such as a tissue or body fluid. The present method is based on the fact that it is possible, through the use of DGGE, to separate nucleic acid sequences which differ by only a single base change and on the ability to detect the separate mutant molecules. The present invention, in another aspect, relates to a method for determining a mutational spectrum in a DNA sequence of interest present in a population of cells. The method of the present invention is useful as a diagnostic or analytical tool in forensic science in assessing environmental and/or occupational exposures to potentially genetically toxic materials (also referred to as potential mutagens); in biotechnology, particularly in the study of the relationship between the amino acid sequence of enzymes and other biologically-active proteins or protein-containing substances and their respective functions; and in determining the effects of drugs, cosmetics and other chemicals for which toxicity data must be obtained.

Inventors:
 [1];  [2]
  1. Winchester, MA
  2. Cambridge, MA
Issue Date:
Research Org.:
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
OSTI Identifier:
867967
Patent Number(s):
5045450
Assignee:
Massachusetts Institute of Technology (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
DOE Contract Number:  
FG02-86ER60448
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
determination; mutational; spectrum; method; resolving; physically; separating; mutant; dna; nonmutant; defining; establishing; profile; alterations; nucleic; acid; sequences; sample; analyzed; tissue; fluid; based; dgge; separate; single; base; change; ability; detect; molecules; aspect; relates; determining; sequence; population; cells; useful; diagnostic; analytical; tool; forensic; science; assessing; environmental; occupational; exposures; potentially; genetically; toxic; materials; referred; potential; mutagens; biotechnology; particularly; study; relationship; amino; enzymes; biologically-active; proteins; protein-containing; substances; respective; functions; effects; drugs; cosmetics; chemicals; toxicity; data; obtained; toxic materials; containing substance; dna sequence; acid sequence; amino acid; nucleic acid; acid sequences; mutational spectrum; mutant dna; physically separating; /435/436/999/

Citation Formats

Thilly, William G, and Keohavong, Phouthone. Determination of a mutational spectrum. United States: N. p., 1991. Web.
Thilly, William G, & Keohavong, Phouthone. Determination of a mutational spectrum. United States.
Thilly, William G, and Keohavong, Phouthone. Tue . "Determination of a mutational spectrum". United States. https://www.osti.gov/servlets/purl/867967.
@article{osti_867967,
title = {Determination of a mutational spectrum},
author = {Thilly, William G and Keohavong, Phouthone},
abstractNote = {A method of resolving (physically separating) mutant DNA from nonmutant DNA and a method of defining or establishing a mutational spectrum or profile of alterations present in nucleic acid sequences from a sample to be analyzed, such as a tissue or body fluid. The present method is based on the fact that it is possible, through the use of DGGE, to separate nucleic acid sequences which differ by only a single base change and on the ability to detect the separate mutant molecules. The present invention, in another aspect, relates to a method for determining a mutational spectrum in a DNA sequence of interest present in a population of cells. The method of the present invention is useful as a diagnostic or analytical tool in forensic science in assessing environmental and/or occupational exposures to potentially genetically toxic materials (also referred to as potential mutagens); in biotechnology, particularly in the study of the relationship between the amino acid sequence of enzymes and other biologically-active proteins or protein-containing substances and their respective functions; and in determining the effects of drugs, cosmetics and other chemicals for which toxicity data must be obtained.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1991},
month = {1}
}