Determination of a mutational spectrum
Abstract
A method of resolving (physically separating) mutant DNA from nonmutant DNA and a method of defining or establishing a mutational spectrum or profile of alterations present in nucleic acid sequences from a sample to be analyzed, such as a tissue or body fluid. The present method is based on the fact that it is possible, through the use of DGGE, to separate nucleic acid sequences which differ by only a single base change and on the ability to detect the separate mutant molecules. The present invention, in another aspect, relates to a method for determining a mutational spectrum in a DNA sequence of interest present in a population of cells. The method of the present invention is useful as a diagnostic or analytical tool in forensic science in assessing environmental and/or occupational exposures to potentially genetically toxic materials (also referred to as potential mutagens); in biotechnology, particularly in the study of the relationship between the amino acid sequence of enzymes and other biologically-active proteins or protein-containing substances and their respective functions; and in determining the effects of drugs, cosmetics and other chemicals for which toxicity data must be obtained.
- Inventors:
-
- Winchester, MA
- Cambridge, MA
- Issue Date:
- Research Org.:
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
- OSTI Identifier:
- 867967
- Patent Number(s):
- 5045450
- Assignee:
- Massachusetts Institute of Technology (Cambridge, MA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- DOE Contract Number:
- FG02-86ER60448
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- determination; mutational; spectrum; method; resolving; physically; separating; mutant; dna; nonmutant; defining; establishing; profile; alterations; nucleic; acid; sequences; sample; analyzed; tissue; fluid; based; dgge; separate; single; base; change; ability; detect; molecules; aspect; relates; determining; sequence; population; cells; useful; diagnostic; analytical; tool; forensic; science; assessing; environmental; occupational; exposures; potentially; genetically; toxic; materials; referred; potential; mutagens; biotechnology; particularly; study; relationship; amino; enzymes; biologically-active; proteins; protein-containing; substances; respective; functions; effects; drugs; cosmetics; chemicals; toxicity; data; obtained; toxic materials; containing substance; dna sequence; acid sequence; amino acid; nucleic acid; acid sequences; mutational spectrum; mutant dna; physically separating; /435/436/999/
Citation Formats
Thilly, William G, and Keohavong, Phouthone. Determination of a mutational spectrum. United States: N. p., 1991.
Web.
Thilly, William G, & Keohavong, Phouthone. Determination of a mutational spectrum. United States.
Thilly, William G, and Keohavong, Phouthone. Tue .
"Determination of a mutational spectrum". United States. https://www.osti.gov/servlets/purl/867967.
@article{osti_867967,
title = {Determination of a mutational spectrum},
author = {Thilly, William G and Keohavong, Phouthone},
abstractNote = {A method of resolving (physically separating) mutant DNA from nonmutant DNA and a method of defining or establishing a mutational spectrum or profile of alterations present in nucleic acid sequences from a sample to be analyzed, such as a tissue or body fluid. The present method is based on the fact that it is possible, through the use of DGGE, to separate nucleic acid sequences which differ by only a single base change and on the ability to detect the separate mutant molecules. The present invention, in another aspect, relates to a method for determining a mutational spectrum in a DNA sequence of interest present in a population of cells. The method of the present invention is useful as a diagnostic or analytical tool in forensic science in assessing environmental and/or occupational exposures to potentially genetically toxic materials (also referred to as potential mutagens); in biotechnology, particularly in the study of the relationship between the amino acid sequence of enzymes and other biologically-active proteins or protein-containing substances and their respective functions; and in determining the effects of drugs, cosmetics and other chemicals for which toxicity data must be obtained.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1991},
month = {1}
}