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Title: Dose critical in-vivo detection of anti-cancer drug levels in blood

Abstract

A method and apparatus are disclosed for the in vivo and in vitro detection and measurement of dose critical levels of DNA-binding anti-cancer drug levels in biological fluids. The apparatus comprises a laser based fiber optic sensor (optrode) which utilizes the secondary interactions between the drug and an intercalating fluorochrome bound to a probe DNA, which in turn is attached to the fiber tip at one end thereof. The other end of the optical fiber is attached to an illumination source, detector and recorder. The fluorescence intensity is measured as a function of the drug concentration and its binding constant to the probe DNA. Anticancer drugs which lend themselves to analysis by the use of the method and the optrode of the present invention include doxorubicin, daunorubicin, carminomycin, aclacinomycin, chlorambucil, cyclophosphamide, methotrexate, 5-uracil, arabinosyl cytosine, mitomycin, cis-platinum 11 diamine dichloride procarbazine, vinblastine vincristine and the like. The present method and device are suitable for the continuous monitoring of the levels of these and other anticancer drugs in biological fluids such as blood, serum, urine and the like. The optrode of the instant invention also enables the measurement of the levels of these drugs from a remote location and from multiplemore » samples.« less

Inventors:
 [1];  [2]
  1. Bethel Island, CA
  2. (late of Livermore, CA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
867744
Patent Number(s):
5001051
Assignee:
Regents of University of California (Oakland, CA)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
dose; critical; in-vivo; detection; anti-cancer; drug; levels; blood; method; apparatus; disclosed; vivo; vitro; measurement; dna-binding; biological; fluids; comprises; laser; based; fiber; optic; sensor; optrode; utilizes; secondary; interactions; intercalating; fluorochrome; bound; probe; dna; attached; tip; optical; illumination; source; detector; recorder; fluorescence; intensity; measured; function; concentration; binding; constant; anticancer; drugs; lend; analysis; doxorubicin; daunorubicin; carminomycin; aclacinomycin; chlorambucil; cyclophosphamide; methotrexate; 5-uracil; arabinosyl; cytosine; mitomycin; cis-platinum; 11; diamine; dichloride; procarbazine; vinblastine; vincristine; device; suitable; continuous; monitoring; serum; urine; instant; enables; remote; location; multiple; samples; continuous monitoring; illumination source; apparatus comprises; fiber optic; optical fiber; remote location; fluorescence intensity; biological fluids; optic sensor; apparatus comprise; biological fluid; multiple samples; multiple sample; anti-cancer drug; drug levels; dose critical; laser based; critical levels; /435/356/385/436/

Citation Formats

Miller, Holly H, and Hirschfeld, deceased, Tomas B. Dose critical in-vivo detection of anti-cancer drug levels in blood. United States: N. p., 1991. Web.
Miller, Holly H, & Hirschfeld, deceased, Tomas B. Dose critical in-vivo detection of anti-cancer drug levels in blood. United States.
Miller, Holly H, and Hirschfeld, deceased, Tomas B. Tue . "Dose critical in-vivo detection of anti-cancer drug levels in blood". United States. https://www.osti.gov/servlets/purl/867744.
@article{osti_867744,
title = {Dose critical in-vivo detection of anti-cancer drug levels in blood},
author = {Miller, Holly H and Hirschfeld, deceased, Tomas B.},
abstractNote = {A method and apparatus are disclosed for the in vivo and in vitro detection and measurement of dose critical levels of DNA-binding anti-cancer drug levels in biological fluids. The apparatus comprises a laser based fiber optic sensor (optrode) which utilizes the secondary interactions between the drug and an intercalating fluorochrome bound to a probe DNA, which in turn is attached to the fiber tip at one end thereof. The other end of the optical fiber is attached to an illumination source, detector and recorder. The fluorescence intensity is measured as a function of the drug concentration and its binding constant to the probe DNA. Anticancer drugs which lend themselves to analysis by the use of the method and the optrode of the present invention include doxorubicin, daunorubicin, carminomycin, aclacinomycin, chlorambucil, cyclophosphamide, methotrexate, 5-uracil, arabinosyl cytosine, mitomycin, cis-platinum 11 diamine dichloride procarbazine, vinblastine vincristine and the like. The present method and device are suitable for the continuous monitoring of the levels of these and other anticancer drugs in biological fluids such as blood, serum, urine and the like. The optrode of the instant invention also enables the measurement of the levels of these drugs from a remote location and from multiple samples.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1991},
month = {1}
}

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