DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Method for packing chromatographic beds

Abstract

Column chromatography beds are packed through the application of static force. A slurry of the chromatography bed material and a non-viscous liquid is filled into the column plugged at one end, and allowed to settle. The column is transferred to a centrifuge, and centrifuged for a brief period of time to achieve a predetermined packing level, at a range generally of 100-5,000 gravities. Thereafter, the plug is removed, other fixtures may be secured, and the liquid is allowed to flow out through the bed. This results in an evenly packed bed, with no channeling or preferential flow characteristics.

Inventors:
 [1];  [2];  [3]
  1. Potomac, MD
  2. Germantown, MD
  3. Rockville, MD
Issue Date:
Research Org.:
University of Maryland (College Park, MD)
OSTI Identifier:
867677
Patent Number(s):
4985143
Assignee:
University of Maryland (College Park, MD)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FG05-85ER13308
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; packing; chromatographic; beds; column; chromatography; packed; application; static; force; slurry; bed; material; non-viscous; liquid; filled; plugged; allowed; settle; transferred; centrifuge; centrifuged; brief; period; time; achieve; predetermined; level; range; 100-5; 000; gravities; thereafter; plug; removed; fixtures; secured; flow; results; channeling; preferential; characteristics; viscous liquid; bed material; packed bed; column chromatography; flow characteristics; chromatography bed; static force; brief period; /210/95/141/

Citation Formats

Freeman, David H, Angeles, Rosalie M, and Keller, Suzanne. Method for packing chromatographic beds. United States: N. p., 1991. Web.
Freeman, David H, Angeles, Rosalie M, & Keller, Suzanne. Method for packing chromatographic beds. United States.
Freeman, David H, Angeles, Rosalie M, and Keller, Suzanne. Tue . "Method for packing chromatographic beds". United States. https://www.osti.gov/servlets/purl/867677.
@article{osti_867677,
title = {Method for packing chromatographic beds},
author = {Freeman, David H and Angeles, Rosalie M and Keller, Suzanne},
abstractNote = {Column chromatography beds are packed through the application of static force. A slurry of the chromatography bed material and a non-viscous liquid is filled into the column plugged at one end, and allowed to settle. The column is transferred to a centrifuge, and centrifuged for a brief period of time to achieve a predetermined packing level, at a range generally of 100-5,000 gravities. Thereafter, the plug is removed, other fixtures may be secured, and the liquid is allowed to flow out through the bed. This results in an evenly packed bed, with no channeling or preferential flow characteristics.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1991},
month = {1}
}