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Title: Method for rapid base sequencing in DNA and RNA

Abstract

A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.

Inventors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Los Alamos, NM
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
OSTI Identifier:
867551
Patent Number(s):
4962037
Assignee:
United States of America (Washington, DC)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; rapid; base; sequencing; dna; rna; provided; fragments; single; fragment; identifiable; bases; suspended; moving; flow; stream; exonuclease; sequentially; cleaves; individual; maintains; cleaved; orderly; train; subsequent; detection; identification; particular; embodiment; forming; individually; tagged; characteristic; fluorescent; dye; excited; fluorescence; output; spectrum; accordingly; sequence; original; reconstructed; output spectrum; particular embodiment; base sequence; flow stream; fluorescent dye; rapid base; base sequencing; subsequent detection; /435/436/

Citation Formats

Jett, James H, Keller, Richard A, Martin, John C, Moyzis, Robert K, Ratliff, Robert L, Shera, E Brooks, and Stewart, Carleton C. Method for rapid base sequencing in DNA and RNA. United States: N. p., 1990. Web.
Jett, James H, Keller, Richard A, Martin, John C, Moyzis, Robert K, Ratliff, Robert L, Shera, E Brooks, & Stewart, Carleton C. Method for rapid base sequencing in DNA and RNA. United States.
Jett, James H, Keller, Richard A, Martin, John C, Moyzis, Robert K, Ratliff, Robert L, Shera, E Brooks, and Stewart, Carleton C. Mon . "Method for rapid base sequencing in DNA and RNA". United States. https://www.osti.gov/servlets/purl/867551.
@article{osti_867551,
title = {Method for rapid base sequencing in DNA and RNA},
author = {Jett, James H and Keller, Richard A and Martin, John C and Moyzis, Robert K and Ratliff, Robert L and Shera, E Brooks and Stewart, Carleton C},
abstractNote = {A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {1}
}