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Title: Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae

Abstract

Chromosomal DNA cassettes containing genes encoding either the DpnI or DpnII restriction endonucleases from Streptococcus pneumoniae are cloned into a streptococcal vector, pLS101. Large amounts of the restriction enzymes are produced by cells containing the multicopy plasmids, pLS202 and pLS207, and their derivatives pLS201, pLS211, pLS217, pLS251 and pLS252.

Inventors:
 [1]
  1. Brookhaven, NY
Issue Date:
Research Org.:
Associated Universities, Inc., Upton, NY (United States)
OSTI Identifier:
867543
Patent Number(s):
4960707
Assignee:
Associated Universities, Inc. (Washington, DC)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
recombinant; plasmids; encoding; restriction; enzymes; dpni; dpnii; streptococcus; pneumontae; chromosomal; dna; cassettes; containing; genes; endonucleases; pneumoniae; cloned; streptococcal; vector; pls101; amounts; produced; cells; multicopy; pls202; pls207; derivatives; pls201; pls211; pls217; pls251; pls252; chromosomal dna; restriction enzyme; cells containing; streptococcus pneumoniae; restriction endonuclease; recombinant plasmids; recombinant plasmid; genes encoding; ii restriction; cells contain; /435/

Citation Formats

Lacks, Sanford A. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae. United States: N. p., 1990. Web.
Lacks, Sanford A. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae. United States.
Lacks, Sanford A. Mon . "Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae". United States. https://www.osti.gov/servlets/purl/867543.
@article{osti_867543,
title = {Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae},
author = {Lacks, Sanford A},
abstractNote = {Chromosomal DNA cassettes containing genes encoding either the DpnI or DpnII restriction endonucleases from Streptococcus pneumoniae are cloned into a streptococcal vector, pLS101. Large amounts of the restriction enzymes are produced by cells containing the multicopy plasmids, pLS202 and pLS207, and their derivatives pLS201, pLS211, pLS217, pLS251 and pLS252.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {1}
}