Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
Abstract
Chromosomal DNA cassettes containing genes encoding either the DpnI or DpnII restriction endonucleases from Streptococcus pneumoniae are cloned into a streptococcal vector, pLS101. Large amounts of the restriction enzymes are produced by cells containing the multicopy plasmids, pLS202 and pLS207, and their derivatives pLS201, pLS211, pLS217, pLS251 and pLS252.
- Inventors:
-
- Brookhaven, NY
- Issue Date:
- Research Org.:
- Associated Universities, Inc., Upton, NY (United States)
- OSTI Identifier:
- 867543
- Patent Number(s):
- 4960707
- Assignee:
- Associated Universities, Inc. (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- recombinant; plasmids; encoding; restriction; enzymes; dpni; dpnii; streptococcus; pneumontae; chromosomal; dna; cassettes; containing; genes; endonucleases; pneumoniae; cloned; streptococcal; vector; pls101; amounts; produced; cells; multicopy; pls202; pls207; derivatives; pls201; pls211; pls217; pls251; pls252; chromosomal dna; restriction enzyme; cells containing; streptococcus pneumoniae; restriction endonuclease; recombinant plasmids; recombinant plasmid; genes encoding; ii restriction; cells contain; /435/
Citation Formats
Lacks, Sanford A. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae. United States: N. p., 1990.
Web.
Lacks, Sanford A. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae. United States.
Lacks, Sanford A. Mon .
"Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae". United States. https://www.osti.gov/servlets/purl/867543.
@article{osti_867543,
title = {Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae},
author = {Lacks, Sanford A},
abstractNote = {Chromosomal DNA cassettes containing genes encoding either the DpnI or DpnII restriction endonucleases from Streptococcus pneumoniae are cloned into a streptococcal vector, pLS101. Large amounts of the restriction enzymes are produced by cells containing the multicopy plasmids, pLS202 and pLS207, and their derivatives pLS201, pLS211, pLS217, pLS251 and pLS252.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1990},
month = {Mon Jan 01 00:00:00 EST 1990}
}
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